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· 분류 : 외국도서 > 과학/수학/생태 > 과학 > 생명과학 > 세포 생물학
· ISBN : 9781118873656
· 쪽수 : 1200쪽
목차
List of Figures, xix
List of Color Plates, xxiii
List of Tables, xxv
List of Protocols, xxvii
List of Minireviews, xxix
Preface and Acknlowledgments, xxxi
Abbreviations, xxxiii
About the Companion Website xxxviii
1. Introduction, 1
2. Biology of Cultured Cells, 19
3. Laboratory Design and Layout, 37
4. Equipment and Materials, 51
5. Aseptic Technique, 73
6. Safety, Bioethics, and Validation, 89
7. Culture Vessels and Substrates, 111
8. Defined Media and Supplements, 125
9. Serum-Free Media, 149
10. Preparation and Sterilization, 173
10. Supplementary Material*
11. Primary Culture, 207
11. Supplementary Material*
12. Subculture and Cell Lines, 235
13. Authentication and Validation, 259
14. Microbial Contamination, 289
15. Cryopreservation and Banking, 307
16. Cloning and Selection, 327
16. Supplementary Material*
17. Cell Sorting, 347
18. Cell Line Characterization, 359
19. Differentiation, 383
20. Threei¿½]Dimensional Culture 401
21. Scalei¿½]up and Automation, 427
22. Senescence, Immortalization, and Transformation, 455
23. Quantitation, 483
23 Supplementary Material*
24. Cytotoxicity, 513
24. Supplementary Material*
25. Culture of Specific Cell Types, 531
25. Supplementary Material*
26. Stem Cells, 571
26. Supplementary Material*
27. Training Programs, 591
28. Problem Solving 627
29. In Conclusion, 651
Supplementary Material*
Appendix III: Sources of Equipment and Material
Appendix IV: Suppliers and Other Resource