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· 제목 : Animal Cell Biotechnology: Methods and Protocols (Paperback, 4, 2020)
· 분류 : 외국도서 > 과학/수학/생태 > 과학 > 생명공학
· ISBN : 9781071601938
· 쪽수 : 399쪽
· 출판일 : 2021-01-17
· 분류 : 외국도서 > 과학/수학/생태 > 과학 > 생명공학
· ISBN : 9781071601938
· 쪽수 : 399쪽
· 출판일 : 2021-01-17
목차
Preface…
Table of Contents…
Contributing Authors…
Part I Cell Line and Media Development
1 Near-Physiological Cell-Cycle Synchronization with Countercurrent Centrifugal Elutriation
Johannes Moller, Katrin Korte, Ralf Portner, An-Ping Zeng, and Uwe Jandt2 Turning Cells into Reagents: The Preparation of Assay Ready Cells for Routine Use in Bioassays
Oliver Wehmeier and Alexander Loa
3 Screening of Media Supplements for High-Performance Perfusion Cultures by Design of Experiment
Patrick Mayrhofer and Renate Kunert
Part II Techniques for Process Development
4 ambr® 15 Microbioreactors for CHO Cell Cultivation
Steve R.C. Warr
5 Using a Parallel Micro-Cultivation System (Micro-Matrix) as a Process Development Tool for Cell Culture Applications
Vincent Wiegmann, Cristina Bernal Martinez, and Frank Baganz
6 HEK293 Cell-Based Bioprocess Development at Bench Scale by Means of On-Line Monitoring in Shake Flasks (RAMOS and SFR)
Tibor Anderlei, Mike Keebler, Jordi Joan Cairo, and Marti Lecina7 Orbitally Shaken Single-Use Bioreactor for Animal Cell Cultivation: Fed-Batch and Perfusion Mode
Tim Burgin, Juliana Coronel, Gerrit Hagens, Michael V. Keebler, Yvonne Genzel, Udo Reichl, and Tibor Anderlei
8 Development of Mammalian Cell Perfusion Cultures at Lab Scale: From Orbitally Shaken Tubes to Benchtop Bioreactors
Moritz Wolf and Massimo Morbidelli
9 Perfusion Control for High Cell Density Cultivation and Viral Vaccine Production
Alexander Nikolay, Thomas Bissinger, Gwendal Granicher, Yixiao Wu, Yvonne Genzel, and Udo Reichl
10 How to Produce mAbs in a Cube-Shaped Stirred Single-Use Bioreactor at 200 L Scale
Cedric Schirmer, Jan Muller, Nina Steffen, Soren Werner, Regine Eibl, and Dieter Eibl
Part III Model-Based Techniques for Process Development
11 Generic Workflow for the Setup of Mechanistic Process Models
Sven Daume, Sandro Kofler, Julian Kager, Paul Kroll, and Christoph Herwig
12 Estimation of Process Model Parameters
Sahar Deppe, Bjorn Frahm, Volker C. Hass, Tanja Hernandez Rodriguez, Kim B. Kuchemuller, Johannes Moller, and Ralf Portner
Kim B. Kuchemuller, Ralf Portner, and Johannes Moller
14 Design, Optimization, and Adaptive Control of Cell Culture Seed Trains
Tanja Hernandez Rodriguez and Bjorn Frahm
Part IV Process Analysis
15 High-Throughput Quantification and Glycosylation Analysis of Antibodies using Bead-Based Assays
Sebastian Giehring
16 Surface Plasmon Resonance-Based Method for Rapid Product Sialylation Assessment in Cell Culture
Olivier Henry, Eric Karengeraa, Florian Cambay, and Gregory De Crescenzo
17 Analysis of Product Quality of Complex Polymeric IgM Produced by CHO Cells
Julia Hennicke and Renate Kunert
Hesham K. Yosef and Karin Schutze
19 An Optical Biosensor for Continuous Glucose Monitoring in Animal Cell Cultures
Mario Lederle, Mircea Tric, Claudio Packi, Tobias Werner, and Philipp Wiedemann
20 Turbidimetry and Dielectric Spectroscopy as Process Analytical Technologies for Mammalian and Insect Cell Cultures
Lukas Kaßer, Jan Zitzmann, Tanja Grein, Tobias Weidner, Denise Salzig, and Peter Czermak
Part V Downstream Techniques
21 Continuous Chromatography Purification of Virus-Based Biopharmaceuticals: A Shortcut Design Method
Ricardo J.S. Silva, Joao Mendes, Manuel J.T. Carrondo, Paula M. Marques, and Cristina Peixoto
22 Single Step Purification of Glycogen Synthase Kinase Isoforms from Small Scale Transient Expression in HEK293 Cells with a Calcium- Dependent Fragment Complementation System
Gavin McGauran, Sara Linse, and David J. O’Connell
