Site-Specific Protein Labeling: Methods and Protocols (Paperback, Softcover Repri)

1. How FlAsH got its Sparkle:Historical Recollections of the Biarsenical-tetracysteine Tag B. Albert Griffin, Stephen R. Adams, and Roger Y. Tsien 2. Site-Specific Protein Labeling in the Pharmaceutical Industry - Experiences from Novartis Drug Discovery Lukas Leder 3. Getting Across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins Nicole J. Yang and Marlon J. Hinner 4. Considerations and Protocols for the Synthesis of Custom Protein Labeling Probes Ivan R. Correa Jr. 5. 2-Cyanobenzothiazole (CBT) Condensation for Site-Specific Labeling of Proteins at the Terminal Cysteine Residues Lina Cui and Jianghong Rao 6. Fluorescent Labeling for Patch Clamp Fluorometry (PCF) Measurements of Real-Time Protein Motion in Ion Channels Thomas K. Berger and Ehud Y. Isacoff 7. Fluorescent Labeling Of SNAP-Tagged Proteins In Cells Grazvydas Lukinavicius, Luc Reymond, and Kai Johnsson 8. HaloTag Technology For Specific And Covalent Labeling Of Fusion Proteins Helene A. Benink and Marjeta Urh 9. Ligation Of Synthetic Peptides To Proteins Using Semisynthetic Protein Trans-Splicing Julian Matern, Anne-Lena Bachmann, Ilka V. Thiel, Gerrit Volkmann, Alexandra Wasmuth, Jens Binschik and Henning D. Mootz 10. Chemical-tag Labeling Of Proteins Using Fully Recombinant Split Inteins Anne-Lena Bachmann, Julian Matern, Vivien Schutz, and Henning D. Mootz 11. Phage Selection Assisted by Sfp Phosphopantetheinyl Transferase Catalyzed Site-Specific Protein Labeling Bo Zhao, Keya Zhang, Karan Bhuripanyo, Yiyang Wang, Han Zhou, Mengnan Zhang, and Jun Yin 12. Site-Specific Biotinylation Of Purified Proteins Using BirA Michael Fairhead and Mark Howarth 13. Site-specific Labeling of Proteins via Sortase: Protocols for the Molecular Biologist Maximilian Wei-Lin Popp 14. BONCAT - Metabolic Labeling, Click Chemistry, and Affinity Purification Of Newly Synthesized Proteomes Peter Landgraf, Elmer R. Antileo, Erin M. Schuman, and Daniela C. Dieterich 15. Genetic Encoding Of Unnatural Amino Acids For Labeling Proteins Kathrin Lang, Lloyd Davis, and Jason W. Chin 16. Labeling Proteins By Affinity-Guided DMAP Chemistry Tomonori Tamura and Itaru Hamachi 17. Ligand-Directed Tosyl Chemistry For Selective Native Protein Labeling In Vitro, In Cells, and In Vivo Shinya Tsukiji and Itaru Hamachi