Advanced Bacterial Genetics: Use of Transposons and Phage for Genomic Engineering: Volume 421 (Hardcover)

Section I. Strain Collections and Genetic Nomenclature.
Chapter 1. Strain Collections and Genetic Nomenclature.

Section II. Transposons.
Chapter 2: Use of Antibiotic Resistant Transposons for Mutagenesis.
Chapter 3: In vivo Mutagenesis Using EZ-Tn5TM.
Chapter 4: Identification of essential genes in bacteria.
Chapter 5: Isolation and use of linked transposons.
Chapter 6: Localized mutagenesis.
Chapter 7: Generation of deletions and duplications using transposons as portable regions of homology with emphasis on Mud and Tn10 transposons.
Chapter 8: Target-Directed Proteolysis in vivo.
Chapter 9: Sets of transposon generated sequence-tagged mutants for structure-function analysis and engineering.
Chapter 10: Using Genomic Microarrays to Study Insertional/Transposon Mutant Libraries.
Chapter 11: Screening Transposon Mutant Libraries Using Full-Genome Oligonucleotide Microarrays.
Chapter 12: Creating recombination-activated genes and sequence-defined mutant libraries using transposons
Chapter 13: Use of transposons to study genetic regulation fusions.
Chapter 14: Genomic screening for regulatory genes using the T-POP transposon.

Section III. Phage.
Chapter 15: Recombineering: in vivo genetic engineering in E. coli, S. enterica and beyond.
Chapter 16: ?U-Red Genetic Engineering in Salmonella enterica serovar Typhimurium.
Chapter 17: Integrase.
Chapter 18: Challenge phage.
Chapter 19: MudP22.
Chapter 20: Phage metagenomics.