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· 분류 : 외국도서 > 의학 > 약리학
· ISBN : 9780123745361
· 쪽수 : 900쪽
목차
- Why Purify Enzymes?
- Strategies and Considerations for Protein Purifications
- Use of Bioinformatics in Planning a Protein Purification
- Preparing A Purification Summary Table
- Setting Up A Laboratory
- Buffers: Principle and Practice
- Measurement of Enzyme Activity
- Quantitation of Protein
- Concentration of Proteins and Removal of Solutes
- Maintaining Protein Stability
- Selecting an Appropriate Method for Expressing a Recombinant Protein
- Bacterial Systems for Production of Heterologous Proteins
- Expression in the Yeast Pichia Pastoris
- Baculovirus-Insect Cell Expression Systems
- Recombinant Protein Production By Transient Gene Transfer Into Mammalian Cells
- Tagging For Protein Expression
- Refolding of Solubilized Inclusion Body Proteins
- Advances In Preparation of Biological Extracts for Protein Purification
- Isolation of Subcellular Organelles and Structures
- Protein Precipitation Techniques
- Affi-Gel Blue for Nucleic Acid Removal and Early Enrichment of Nucleotide Binding Proteins
- Ion Exchange Chromatography
- Gel Filtration
- Protein Chromatography on Hydroxyapatite Columns
- Theory and Use of Hydrophobic Interaction Chromatography in Protein Purification Applications
- Affinity Chromatography: General Methods
- Immobilized-Metal Affinity Chromatography (IMAC)
- Identification, Production, and Use of Polyol-Responsive Monoclonal Antibodies for Immunoaffinity Chromatography
- One-Dimensional Gel Electrophoresis
- Protein Analysis Using High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis and Isoelectric Focusing
- Protein Gel Staining Methods: An Introduction And Overview
- Elution of Proteins From Gels
- Immunodetection By Protein Blotting Performing and Optimizing Western Blots With An Emphasis on Chemiluminescent Detection
- Detergents: An Overview
- Purification of Membrane Proteins
- Purification of Recombinant G-Protein-Coupled Receptors
- Cell-Free Translation Of Integral Membrane Proteins Into Unilamelar Liposomes
- Determination of Protein Purity
- Determination of Size, Molecular Weight, and Presence of Subunits
- Identification and Quantification of Protein Posttranslational Modifications
- Parallel Methods For Expression And Purification
- Techniques To Isolate O2-Sensitive Proteins: [4Fe-4S]-FNR As An Example
- Rethinking Your Purification Procedure
- Important But Little Known (Or Forgotten) Artifacts in Protein Biochemistry
Murray Deutscher and Arthur Kornberg
Section I: Developing Purification Procedures
Stuart Linn
Richard R. Burgess
Richard R. Burgess
Section II: General Methods for Handling Proteins and Enzymes
Murray P. Deutscher
Vincent S. Stoll and John S. Blanchard
T. K. Harris and M. M. Keshwani
James E. Noble and Marc J. A. Bailey
Jonathan K. Romero, David R. H. Evans and Matthew Westoby
Murray P. Deutscher
Section III: Recombinant Protein Expression and Purification
William H. Brondyk
Frank R. Collart, Ashley M. Frank and Sarah Zerbs
James M. Cregg, Ilya Tolstorukov, Anasua Kusari, Jay Sunga, Knut Madden and Thomas Chappell
Donald L. Jarvis
Sabine Geisse and Cornelia Fux
Arun Malhotra
Richard R. Burgess
Section IV: Preparation of Extracts and Subcellular Fractionation
Anthony C. Grabski
Jorg Von Hagen and Uwe Michelsen
Section V: Purification Procedures: Bulk Methods
Richard R. Burgess
Murray P. Deutscher
Section VI: Purification Procedures: Chromatographic Methods
Alois Jungbauer and Rainer Hahn
Earle Stellwagen
Larry J. Cummings, Mark A. Snyder and Kimberly Brisack
Justin T. Mccue
Section VII: Purification Procedures: Affininty Methods
Marjeta Urh, Dan Simpson and Kate Zhao
Frank Schafer* Helena Block, Barbara Maertens, Anne Spriestersbach, Nicole Brinker, Jan Kubicek, Roland Fabis And Jorg Labahn
Nancy E. Thompson, Katherine M. Foley, Elizabeth S. Stalder, And Richard R. Burgess
Section VIII: Purification Procedures: Electrophoretic Methods
David E. Garfin
Friedman
Thomas H. Steinberg
Richard R. Burgess
Alice Alegria-Schaffer, Krishna Vattem and Andrew Lodge
Section IX: Purification Procedures: Membrane Proteins And Glycoproteins
Dirk Linke
Sue-Hwa Lin and Guido Guidotti
Reinhard Grisshammer
Michael A. Goren, Akira Nozawa, Shin-Ichi Makino, Russell L. Wrobel and Brian G. Fox
Section X: Characterization of Purified Proteins
David G. Rhodes and Thomas M. Laue
David G. Rhodes, Robert E. Bossio and Thomas M. Laue
Andrew Link
Scott A. Lesley
Aixin Yan and Patricia J. Kiley
Murray Deutscher
Richard R. Burgess