RNA Methodologies: A Laboratory Guide for Isolation and Characterization (Paperback, 4)

**Fully revised with five new chapters, a new appendix, and nine chapters with major expansions**

CHAPTER 1: RNA and the Cellular Biochemistry Revisited
CHAPTER 2: RNA Isolation Strategies
CHAPTER 3: The Truth about Tissues
CHAPTER 4: Going Green: RNA and the Molecular Biology of Plants (NEW)
CHAPTER 5: Isolation of Polyadenylated RNA
CHAPTER 6: Quality Control for RNA Preparations (EXPANDED)
CHAPTER 7: Resilient Ribonucleases
CHAPTER 8: Stringency: Conditions that Influence Nucleic Acid Structure (EXPANDED)
CHAPTER 9: Electrophoresis of RNA (EXPANDED)
CHAPTER 10: Photodocumentation and Image Analysis (EXPANDED)
CHAPTER 11: Northern Analysis
CHAPTER 12: Nucleic Acid Probe Technology
CHAPTER 13: Practical Nucleic Acid Hybridization
CHAPTER 14: Principles of Detection (EXPANDED)
CHAPTER 15: Quantification of Specific mRNAs by Nuclease Protection
CHAPTER 16: Analysis of Nuclear RNA (EXPANDED)
CHAPTER 17: cDNA Synthesis (EXPANDED)
CHAPTER 18: RT-PCR (NEW)
CHAPTER 19: Quantitative PCR Techniques
CHAPTER 20: Functional Genomics and Transcript Profiling (NEW)
CHAPTER 21: High-Throughput Analysis of Gene Expression
CHAPTER 22: Non-Array Methods for Global Analysis of Gene Expression (NEW)
CHAPTER 23: RNA Interference: Targeted Gene Silencing (EXPANDED)
CHAPTER 24: Genomes, Transcriptomes, Proteomes, and Bioinformatics
CHAPTER 25: An RNA Paradigm

APPENDIX A: Maintaining Complete and Accurate Records
APPENDIX B: Converting Mass to Moles (NEW)
APPENDIX C: Useful Stock Solutions for the Molecular Biologist
APPENDIX D: Phenol Preparation
APPENDIX E: Disposal of Ethidium Bromide and SYBR Green Solutions
APPENDIX F: DNase I Removal of DNA From An RNA Sample
APPENDIX G: DNase Incubation to Remove RNA from a DNA Sample
APPENDIX H: Deionization of Formamide, Formaldehyde, and Glyoxal
APPENDIX I: Silanizing Centrifuge Tubes and Glassware
APPENDIX J: Trypsinization Protocol for Anchorage-Dependent Cells
APPENDIX K: Isolation of High-Molecular-Weight DNA by Salting-Out
APPENDIX L: Electrophoresis: Principles, Parameters, and Safety
APPENDIX M: Polyacrylamide Gel Electrophoresis
APPENDIX N: Dot Blot Analysis (NEW)
APPENDIX O: Centrifugation as a Mainstream Tool for the Molecular Biologist
APPENDIX P: Selected Suppliers of Equipment, Reagents, and Services
APPENDIX Q: Useful SI Units
APPENDIX R: Common Abbreviations
APPENDIX S: Trade Citations