Myoblast Transfer Therapy (Hardcover, 1990)

1 Molecular Biology of Myogenesis and Regeneration.- MyoD: A Regulatory Gene of Skeletal Myogenesis.- Myoblasts, Satellite Cells, and Myoblast Transfer.- Regeneration of Skeletal Muscle Fibers after Necrosis.- Detection of Truncated Dystrophin in Fetal DMD Myotubes.- General Discussion.- 2 Myoblast/Satellite Cell Antigenicity.- Immunological Aspects of Histoincompatible Myoblast Transfer into Non-tolerant Hosts.- A Comparison of Long-term Survival of Muscle Precursor Cell Suspensions and Minced Muscle Allografts in the Non-tolerant Mouse.- N-CAM is a Target Cell Surface Antigen for the Purification of Muscle Cells for Myoblast Transfer Therapy.- Immunochemical Analyses of the Myoblast Membrane and Lineage.- General Discussion.- 3 Practical Aspects of Myoblast Implantation.- The Principles and Practice of Myoblast Transfer.- Myoblast Transfer Improves Muscle Genetics/Structure/ Function and Normalizes the Behavior and Life Span of Dystrophic Mice.- Practical Aspects of Myoblast Implantation: Investigations on Two Inherited Myopathies in Animals.- Purification and Proliferation of Human Myoblasts Isolated with Fluorescence Activated Cell Sorting.- The Proliferation and Fusion of Myoblasts In Vivo.- The DMD Gene Promoter: A Potential Role in Gene Therapy.- General Discussion.- 4 In Situ Fusion: Nuclear Domains and mRNA/Protein Migration.- Fibroblasts Fuse with Myotubes Developing in Culture.- Phenotypic and Functional Reversion of Muscular Dysgenesis by Heterotypic Fibroblast-Myotube Fusion In Vitro.- Control of Satellite Cell Proliferation.- Regeneration of Skeletal Muscle Induced by Satellite Cell Grafts.- Localization of Muscle Gene Products in Nuclear Domains: Does this Constitute a Problem for Myoblast Therapy?.- Mouse Chimeras and Genetic Rescue of Mosaic Muscle.- General Discussion.- 5 Development of Myogenic Cell Cultures.- Improved Media for Rapid Clonal Growth of Normal Human Skeletal Muscle Satellite Cells.- Retroviral Lineage Markers for Assessing Myoblast Fate In Vivo.- Myogenic Conversion of Human Non-muscle Cells for the Diagnosis and Therapy of Neuromuscular Diseases.- General Discussion.- 6 Monitoring Clinical Success: Phenotypic Transformation.- Genetic and Biochemical Determinations in the Pre-transplant Workup and in the Post-transplant Assessment Period.- Use of Quantitative Myometry in the Evaluation of Myoblast Transfer Therapy.- Clinical Issues in Myoblast Transfer.- Quantitation of Muscle Mass and Muscle Protein Synthesis Rate: Documenting a Response to Myoblast Transfer.- Plausible Structural/Functional/Behavioral/Biochemical Transformations Following Myoblast Transfer Therapy.- Mdx Mouse as Therapeutic Model System: Development and Implementation of Phenotypic Monitoring.- Golden Retriever Muscular Dystrophy: Monitoring for Success.- Molecular Markers for Myoblast Transplantation in GRMD.- The Role of the Xmd Dog in the Assessment of Myoblast Transfer Therapy.- The Dilemma of Manifesting Carriers in the Context of Myoblast Transplantation.- Immunosuppressive Therapy in Duchenne Muscular Dystrophy: Considerations for Myoblast Transfer Studies.- General Discussion.- 7 Implementation of Human Trials.- Synopsis of Proceedings of the Workshop on the Implementation of Myoblast Transfer in Humans.- Participants.