Engineering Complex Phenotypes in Industrial Strains (Hardcover)

Foreword vii
John Pierce

Preface ix

Contributors xi

1 Classical Strain Improvement 1
Nathan Crook and Hal S. Alper

2 Tracer-Based Analysis of Metabolic Flux Networks 35
Michael Dauner

3 Integration of “Omics” Data with Genome-Scale Metabolic Models 77
Stephen Van Dien, Priti Pharkya, and Robin Osterhout

4 Strain Improvement via Evolutionary Engineering 111
Byoungjin Kim, Jing Du, and Huimin Zhao

5 Rapid Fermentation Process Development and Optimization 133
Jun Sun and Lawrence Chew

6 The Clavulanic Acid Strain Improvement Program at DSM Anti-Infectives 169
Bert Koekman and Marcus Hans

7 Metabolic Engineering of Recombinant E. coli for the Production of 3-Hydroxypropionate 185
Tanya Warnecke Lipscomb, Matthew L. Lipscomb, Ryan T. Gill, and Michael D. Lynch

8 Complex System Engineering: A Case Study for an Unsequenced Microalga 201
Michael T. Guarnieri, Lieve M.L. Laurens, Eric P. Knoshaug, Yat-Chen Chou, Bryon S. Donohoe, and Philip T. Pienkos

9 Meiotic Recombination-Based Genome Shuffling of Saccharomyces Cerevisiae and Schefferomyces Stiptis for Increased Inhibitor Tolerance to Lignocellulosic Substrate Toxicity 233
Dominic Pinel and Vincent J.J. Martin

Index 251