Plant Pathology: Method and Protocols (Hardcover)

Dedication…
Acknowledgement…
Preface…
Table of Contents…
Contributing Authors…

Part I Plant Disease Diagnosis: Traditional Methods

1. Obtaining and Maintaining Cultures of Pinewood Nematodes Bursaphelenchus xylophilus from Wild Dauers
Carla S. Pimentel, Paulo N. Firmino, and Matthew P. Ayres

2. Survey and Monitoring of Phytophthora Species in Natural Ecosystems: Methods for Sampling, Isolation, Purification, Storage, and Pathogenicity Tests
Ana Perez-Sierra, Marilia Horta Jung, and Thomas Jung

3. Field and Laboratory Procedures for Fusarium circinatum Identification and Diagnosis
Cristina Zamora-Ballesteros, Reinaldo Pire, and Julio Javier Diez

Part II DNA Extraction Protocols

4. Rapid Extraction of Plant Nucleic Acids by Microneedle Patch for In-Field Detection of Plant Pathogens
Rajesh Paul, Emily Ostermann, and Qingshan Wei

5. DNA Extraction Methods to Obtain High DNA Quality from Different Plant Tissues
Domenico Rizzo, Daniele Da Lio, Linda Bartolini, Cristina Francia, Antonio Aronadio, Nicola Luchi, Sara Campigli, Guido Marchi, and Elisabetta Rossi

6. A Rapid Method for Extracting High Quality DNA from Soils
Anna Maria Vettraino and Nicola Luchi

Part III Molecular Methods to Detect Plant Pathogens

7. Invasive Alien Plant Pathogens: The Need of New Detection Methods
Alberto Santini and Duccio Migliorini

8. Detection of Airborne Inoculum of Hymenoscyphus fraxineus: The Causal Agent of Ash Dieback
Milo? Dvo?ak

9. Molecular Detection of Wheat Blast Pathogen in Seeds
Renaud Ioos

10. Detection and Identification of the Causal Agents of Dothistroma Needle Blight
Funda Oskay, Asko Lehtijarvi, and Hatice Tu?ba Do?mu? Lehtijarvi

11.  The Use of qPCR to Detect Cryphonectria parasitica in Plants
Anne Chandelier

12. Rapid Molecular Diagnostics in the Field and Laboratory to Detect Plant Pathogen DNA in Potential Insect Vectors
Karolina Pusz-Bochenska, Edel Perez-Lopez, Tim J. Dumonceaux, Chrystel Olivier, and Tyler J. Wist

13. A Panel of Real-Time PCR Assays for the Direct Detection of All the Xylella fastidiosa Subspecies
Jennifer Hodgetts

14. Selective Quantification of Erwinia amylovora Live Cells in Pome Fruit Tree Cankers by Viability Digital PCR
Ricardo Delgado Santander, Katarina Ga?i?, and Srđan Goran A?imovi?

15. Innovative Detection of the Quarantine Plant Pathogen Curtobacterium flaccumfaciens pv. flaccumfaciens, Causal Agent of Bacterial Wilt of Leguminous Plants
Stefania Tegli, Dario Gaudioso, and Damiano Stefanucci

16. Specific Detection of Pseudomonas savastanoi pathovars: From End-Point PCR to Real-Time PCR and HRMA
Stefania Tegli and Chiara Pastacaldi

Part IV High-Throughput Sequencing

17. Computational Analysis of HTS Data and Its Application in Plant Pathology
Edoardo Piombo and Mukesh Dubey

18. Biomonitoring of Fungal and Oomycete Plant Pathogens by Using Metabarcoding
Emilie D. Tremblay and Guillaume J. Bilodeau

19. Phytobiome Metabarcoding: A Tool to Help Identify Prokaryotic and Eukaryotic Causal Agents of Undiagnosed Tree Diseases
Carrie J. Fearer, Antonino Malacrino, Cristina Rosa, and Pierluigi Bonello

Part V Plant-Pathogen Interactions

20. Plant-Fungal Interactions: Laser Microdissection as a Tool to Study Cell Specificity
Raffaella Balestrini and Fabiano Sillo

21. Somatic Embryogenesis as a Tool for Studying Grapevine-Virus Interaction
Giorgio Gambino and Irene Perrone

22. A Quantitative Luminol-Based Assay for ROS Burst Detection in Potato Leaves in Response to Biotic Stimuli
Muhammad Awais Zahid, Ramesh R. Vetukuri, and Erik Andreasson

Part VI Population Genomics in Plant Pathology

23. Genetic Analysis of Plant Pathogens Natural Populations
Fabiano Sillo

24. Microsatellite Genotyping in the Chestnut Blight Fungus Cryphonectria parasitica
Quirin Kupper and Simone Prospero

25. A Multiplex PCR Approach to Determine Vegetative Incompatibility Genotypes and Mating Type in Cryphonectria parasitica
Quirin Kupper and Carolina Cornejo

Part VII Biocontrol

26. A Detached Leaf Assay for Rapidly Screening Plant Pathogen-Biological Control Agents
Giovana Prado Fortuna Macan, Sammar Khalil, Pruthvi B. Kalyandurg, Nidhi Pareek, and Ramesh R. Vetukuri

27. Spray-Induced Gene Silencing to Study Gene Function in Phytophthora
Poorva Sundararajan, Pruthvi B. Kalyandurg, Qinsong Liu, Aakash Chawade, Stephen C. Whisson, and Ramesh R. Vetukuri

28. Plant Sampling for Production of Essential Oil and Evaluation of Its Antimicrobial Activity In Vitro
Felicia Menicucci, Eleonora Palagano, Aida Raio, Gabriele Cencetti, Nicola Luchi, Andrea Ienco, and Marco Michelozzi

Subject Index List…