Mitochondrial DNA: Methods and Protocols (Hardcover, 2023)

· 제목 : Mitochondrial DNA: Methods and Protocols (Hardcover, 2023) 
· 분류 : 외국도서 > 과학/수학/생태 > 과학 > 생명과학 > 유전학/유전체학
· ISBN : 9781071629215
· 쪽수 : 469쪽
· 출판일 : 2023-02-21

 

Section I. Purification methods for mitochondrial DNA

 

1          Isolation of functional mitochondria and pure mtDNA from murine tissues

            Dieu Hien Rozsivalova, Milica Popovic, Harshita Kaul and Aleksandra Trifunovic

 

2          Isolating mitochondria, mitoplasts, and mtDNA from cultured mammalian cells

            Katja E. Menger and Thomas J. Nicholls

 

3          Coupling differential centrifugation with exonuclease treatment and size exclusion       chromatography (DIFSEC) for purification of mtDNA from mammalian cells

            Andrew M. Shaw and Payam A. Gammage

 

4          Isolation and Quality Control of Yeast Mitochondria

            Asli Aras Taskin, Daiana Nerina Moretti, F.-Nora Vogtle and Chris Meisinger

 

5          Mitochondrial DNA isolation from plants

            Frederique Weber-Lotfi, Arnaud Fertet, Rokas Kubilinskas, Clementine Wallet and Jose M.          Gualberto

 

Section II. Visualising mtDNA

 

6          Visualize the Distribution and Dynamics of Mitochondrial DNA (mtDNA) nucleoids with        Multiple Labelling Strategies

            Xiangjun Di, Jinshan Qin, Yujie Sun, Qian Peter Su

 

7          Visualization of mtDNA using FISH

            Xie Xie and Xuefeng Zhu

 

8          In situ analysis of mitochondrial DNA synthesis using metabolic labeling coupled to     fluorescence microscopy

            John A. Smolka and Samantha C. Lewis

 

9          Measurement of nucleoid size using STED microscopy

            Elisa Motori

 

Section III. Mitochondrial DNA interacting proteins

 

10        How to quantify DNA compaction by TFAM with Acoustic Force Spectroscopy and Total          Internal Reflection Fluorescence microscopy

            Martucci Martial, Debar Louis, van den Wildenberg Siet and Farge Geraldine

 

11        Assessing TFAM binding to human mitochondrial DNA

            Takehiro Yasukawa and Dongchon Kang

 

12        Identification of proximity interactors of mammalian nucleoid proteins by BioID

            Mari J. Aaltonen and Hana Antonicka

 

13        Localization of mitochondrial nucleoids by transmission electron microscopy using the transgenic expression of the mitochondrial helicase TWINKLE and APEX2

            David Pla-Martin, Felix Babatz, Astrid Schauss

 

Section IV. Mitochondrial DNA replication and repair

 

14        In vitro assays of TWINKLE function

            Jay P. Uhler, Ulrika Alexandersson and Maria Falkenberg

 

15        Rolling Circle Replication and Bypass of Damaged Nucleotides

            Josefin M. E. Forslund, Gorazd Stojkovi? and Sjoerd Wanrooij

 

16        Studying mitochondrial nucleic acid synthesis utilizing intact isolated mitochondria

            Jelena Misic and Dusanka Milenkovic

 

17        Functional assessment of mitochondrial DNA maintenance by depletion and repopulation     using 2’,3’-dideoxycytidine in cultured cells

            Gabor Zsurka, Genevieve Trombly, Susanne Scholer, Daniel Blei, Wolfram S. Kunz

 

18        Analysis of mitochondrial DNA replication by two-dimensional agarose gel electrophoresis

            Steffi Goffart and Jaakko Pohjoismaki

 

19        Quantitative analysis of nucleoside triphosphate pools in mouse muscle using hydrophilic        interaction liquid chromatography coupled with tandem mass spectrometry detection

            Sushma Sharma, Ziqing Kong, Shaodong Jia, Phong Tran, Anna Karin Nilsson, and Andrei      Chabes

 

20        Detection of UV-Induced Deletions in Mitochondrial DNA

            Gabriele A. Fontana and Hailey L. Gahlon

 

21        Determination of the ribonucleotide content of mtDNA using alkaline gels

            Choco Michael Gorospe, Bruno Marcal Repoles and Paulina H. Wanrooij

 

22        5´-end mapping in human mitochondrial DNA

            Andranik Durgaryan, Anders R. Clausen

 

Section V. Modifying mitochondrial DNA

 

23        Manipulation of murine mitochondrial DNA heteroplasmy with mtZFNs

            Pavel A. Nash and Michal Minczuk

 

24        Biolistic transformation of Chlamydomonas reinhardtii and Saccharomyces cerevisiae      mitochondria

            Nathalie Bonnefoy and Claire Remacle

 

25        A method for precisely identifying modifications to plant mitochondrial genomes by     mitoTALENs

            Tomohiko Kazama and Shin-ichi Arimura

 

Section VI. Mitochondrial DNA in human disease

 

26        Mitochondrial DNA sequencing and heteroplasmy quantification by Next Generation     Sequencing

            Andrea Legati, Daniele Ghezzi and Carlo Viscomi

 

27        Genomic strategies in mitochondrial diagnostics

            Dasha Deen, Charlotte L. Alston, Gavin Hudson, Robert W. Taylor, Angela Pyle

 

28        Mitochondrial DNA enrichment for sensitive next-generation sequencing

            Shilan Wu, Matthew J. Longley, Scott A. Lujan, Thomas A. Kunkel and William C. Copeland

 

29        Single cell analysis of mitochondrial DNA deletions

            Helen A.L. Tuppen, Amy K. Reeve, Amy E. Vincent