Gc-MS of Biologically and Environmentally Significant Organic Compounds: Tms Derivatives (Hardcover)

Preface vii

1 Introduction 1

2 Gas Chromatography and Mass Spectrometry Considerations 7

2.1 Silylation Reagents and Procedure of Derivatisation 7

2.2 Two-Step Derivatisation: Oximating−Silylating 8

2.3 Analytical Procedure 10

2.3.1 Selection of Stationary Phase and Conditions of Separation 10

2.3.2 Sample Preparation 10

2.3.3 Qualitative MS Identification 12

2.3.3.1 Aliphatic Alcohols 16

2.3.3.2 Aromatic Alcohols 16

2.3.3.3 Carboxylic Acids 16

2.3.3.4 Amino Acids 16

2.3.3.5 Phenylpropanoids 17

2.3.3.6 Flavonoids 18

2.3.3.7 Steroids 20

2.3.3.8 Carbohydrates and Glycosides 22

2.4 A Critical Role of Retention Indices in GS–MS Investigations 24

2.5 Experimental Factors Influencing the Accuracy of Retention Indices 28

2.6 Possible Artefacts and Complications of Mass Spectral Identification 30

3 Explanation of Format Used in the Book 33

4 References 35

5 Alphabetic Listing of Compounds 43

6 Compounds Listed in Order of Their Retention Indices 87

7 Mass Spectra and Retention Indices of TMS Derivatives 131