Modern Clinical Molecular Techniques (Hardcover, 2012)

Preface Acknowledgement Contributors Section I Current Molecular Laboratory Methods 1 Specimen Collection, Handling and Processing Lindsy Hengesbach and John Gerlach 2 DNA/RNA Isolation and Quantitation Jing Wang 3 Test Validation Cindy McCloskey and S Terence Dunn 4 In Vitro Amplification Methods in Molecular Diagnostics C. Alexander Valencia and Bradford Coffee 5 Whole Genome Sequencing in the Clinical Laboratory Tina Hambuch, Brad Sickler, Arnold Liao, Suneer Jain, and Philip D. Cotter 6 Estimation of Exon Dosage Using Real-Time Quantitative Polymerase Chain Reaction Juliana Purrazzella and Arupa Ganguly 7 Reporting Clinical Molecular Genetic Laboratory Results Victor Wei Zhang and Lora J. H. Bean Section II Clinical Molecular Infectious Disease Testing 8 HIV-1 Genotyping and Virtual Phenotyping Fangling Xu and Jianli Dong 9 COBAS® Ampliprep/Taqman HIV-1 Test Tavat A. Buraruk and Charles E. Stager 10 Hepatitis C Virus (HCV) Genotype Assay (LiPA) Charles E. Stager and Tavat A. Buraruk 11 COBAS® Ampliprep/Taqman HCV Test Charles E. Stager and Tavat A. Buraruk 12 Epstein-Barr Virus and Cytomegalovirus Viral Load Monitoring by Quantitative Real-Time Polymerase Chain Reaction Su S. Chen, Seema Hai, and Rajyalakshmi Luthra 13 Quantification of BK Viral Load Hai Wu and Jianli Dong 14 Molecular Diagnosis and Epidemiology of Rabies Rodney E. Rohde and Bonny C. Mayes 15 Pyrosequencing for Bacterial Identification Laura Millecker and Ruth Ann Luna 16 Other Pathogens of Significant Public Health Concern Chris L. McGowin, Rodney E. Rohde, and Greg C. Whitlock Section III Clinical Molecular Oncology Testing 17 Gene Rearrangements Testing Siby Sebastian, Juan Du, and Rizwan Naeem 18 Mutation Analysis in Cancer Keyur Pravinchandra Patel, John Galbincea, and Rajyalakshmi Luthra 19 Minimal Residual Disease Wesley Greaves and Rajyalakshmi Luthra 20 Pyrosequencing in Cancer Bedia A. Barkoh, Rajyalakshmi Luthra, and Shalini Verma 21 Clinical Applications of Microarrays in Cancer Marilyn M. Li, Ankita Patel, and Xiaofeng Hu 22 Basic Theories and Clinical Applications of Molecular Flow Cytometry Hua Kang Wu, Hongli Chen, and Peter Hu Section IV Clinical Molecular Pre/Postnatal Testing 23 Molecular Prenatal Diagnostic Testing Joanna Wiszniewska and Christine M. Eng 24 PCR Based Diagnosis of Fragile X Syndrome Daniel J. Dykas and Allen E. Bale Section V Human Identity Testing 25 Microsatellite Testing Short Tandem Repeat Profile Generation Robin DeVille Guidry and Laura Gahn 26 Parentage Laura Gahn and Robin DeVille Guidry Section VI Clinical Molecular Training 27 Diagnostic Molecular Training Peter Hu, Patrick A. Lennon, and Madhuri Hegde 28 Molecular Diagnostics Point of Care Testing Training Chang W. Lee, and Peter Hu