Molecular Engineering: Principles and Applications (Hardcover)

Fundamentals. Biological Information Flow Replication, Transcription, Translation, Molecular Players. Structure and Function of Nucleic Acids and Proteins, Components and Physical/Chemical Properties, Structure Determination: X-ray Crystallography & NMR. Practice in Molecular Engineering and Validation. DNA Recombinant Technology Primer Design, Vector Design. Rational Design of Molecules, Structure and Function, Force Fields, Computational Tools. Directed Evolution Platforms Phage, Yeast, SELEX: Aptamers. Techniques for Molecular Interaction Studies, SPR, ITC, Gel Filtration, Mass-Spec, Gel Shift (EMSA). Applications. Antibody Engineering (structure, function etc.). Virus Engineering, Gene Delivery by Virus and Virus-Like Particles. Application to Diagnosis and Therapy, Whole Body Imaging Techniques: MRI, CT, PET, Optical Imaging, Conjugation Chemistry for Therapy and Diagnosis, Engineering Considerations for PK and PD, Immunotherapy.