Cancer Cell Signaling: Methods and Protocols (Hardcover, 2, 2014)

Part I: Targeting Cancer Cell Metabolism as Anticancer Therapy 1. Use of Biguanides to Improve Response to Chemotherapy Vlad C. Sandulache, Liangpeng Yang, and Heath D. Skinner 2. Evaluating Response to Metformin/Cisplatin Combination in Cancer Cells Via Metabolic Measurement and Clonogenic Survival Sang Hyeok Woo, Vlad C. Sandulache, Liangpeng Yang, and Heath D. Skinner 3. Quantifying the Autophagy-Triggering Effects of Drugs in Cell Spheroids with Live Fluorescence Microscopy Nariman Ansari, Stefanie Muller, Katharina Hotte, Stefanie Rakel, Patrick Antonietti, Donat Kogel, Ernst H.K. Stelzer, and Francesco Pampaloni Part II: Epigenetic Control of Cancer 4. Interdependency Between Genetic and Epigenetic Regulatory Defects in Cancer Felix Recillas-Targa 5. Experimental Strategies to Manipulate the Cellular Levels of the Multifunctional Factor CTCF Edgar Gonzalez-Buendia, Rosario Perez-Molina, Erandi Ayala-Ortega, Georgina Guerrero, and Felix Recillas-Targa 6. Use of Serum-Circulating miRNA Profiling for the Identification of Breast Cancer Biomarkers Fermin Mar-Aguilar, Cristina Rodriguez-Padilla, and Diana Resendez-Perez 7. Prostate Cancer Detection Using a Non-Invasive Method for Quantifying miRNAs Mauricio Rodriguez-Dorantes, A. Ivan Salido-Guadarrama, and Pilar Garcia-Tobilla 8. DNA Methylation Analysis of Steroid Hormone Receptor Genes Ignacio Camacho-Arroyo, Valeria Hansberg-Pastor, and Mauricio Rodriguez-Dorantes 9. Control of Oncogenic miRNA Function by Light-Activated miRNA Antagomirs Colleen M. Connelly and Alexander Deiters 10. Methods for the Study of Long Non-Coding RNA in Cancer Cell Signaling Yi Feng, Xiaowen Hu, Youyou Zhang, Dongmei Zhang, Chunsheng Li, and Lin Zhang Part III: Metastasis Promotion 11. Microvesicles as Mediators of Intercellular Communication in Cancer Marc A. Antonyak and Richard A. Cerione 12. In Vivo Rat Model to Study Horizontal Tumor Progression Catalina Trejo-Becerril, Enrique Perez-Cardenas, and Alfonso Duenas-Gonzalez 13. An In Vivo Model to Study the Effects of Tumoral Soluble Factors on the Vascular Permeability in Mice Cesar Alejandro Guzman-Perez, Alfredo Ibarra-Sanchez, Jose Luis Ventura-Gallegos, Claudia Gonzalez-Espinosa, Jonathan Garcia-Roman, and Alejandro Zentella-Dehesa 14. Use of the Tumor Repressor DEDD as a Prognostic Marker of Cancer Metastasis Qi Lv, Fang Hua, and Zhuo-Wei Hu 15. Zebrafish-Based Systems Pharmacology of Cancer Metastasis Yasuhito Shimada, Yuhei Nishimura, and Toshio Tanaka Part IV: New Technologies in the Study of Cancer 16. A Flow Cytometry-Based Assay for the Evaluation of Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) in Cancer Cells Nohemi Salinas-Jazmin, Emiliano Hisaki-Itaya, and Marco A. Velasco-Velazquez 17. Gene Disruption Using Zinc Finger Nuclease Technology Sara Granja, Ibtissam Marchiq, Fatima Baltazar, and Jacques Pouyssegur