RNA Nanotechnology and Therapeutics: Methods and Protocols (Hardcover, 2015)

1. Overview of Methods in RNA Nanotechnology - Synthesis, Purification and Characterization of RNA Nanoparticles Farzin Haque and Peixuan Guo 2. Multiple Approaches for the Investigation of Bacterial Small Regulatory RNAs Self-Assembly Christophe Lavelle, Florent Busi, and Veronique Arluison 3. Measuring the Elasticity of Ribonucleotide(s)-Containing DNA Molecules Using AFM Kyung Duk Koh, Hsiang-Chih Chiu, Elisa Riedo, and Francesca Storici 4. Silver Nanoclusters for RNA Nanotechnology: Steps Towards Visualization and Tracking of RNA Nanoparticle Assemblies Kirill A. Afonin, Danielle Schultz, Luc Jaeger, Elisabeth Gwinn, and Bruce A. Shapiro 5. Purification of RNA Nanoparticles by Ultracentrifugation Daniel L. Jasinski, Farzin Haque, and Peixuan Guo 6. HPLC Purification of RNA Aptamers up to 59 Nucleotides with Single Nucleotide Resolution Zhen Huang, Chi-yen Lin, William Jaremko, and Li Niu 7. RNA Nanoparticles with Thermostable Motifs and Fluorogenic Modules for Real-Time Detection of RNA Folding and Turnover In Vivo Hui Zhang, Fengmei Pi, Dan Shu, Mario Vieweger, and Peixuan Guo 8. Fluorescence Labeling of Short RNA by Oxidation at the 3'-End Chen Qiu, Wang-Yi Liu, and Yong-Zhen Xu 9. Methods and Assays for Specific Targeting and Delivery of RNA Nanoparticles to Cancer Metastases Piotr Rychahou, Yi Shu, Farzin Haque, Jiyao Hu, Peixuan Guo, and B. Mark Evers 10. Functional Assays for Specific Targeting and Delivery of RNA Nanoparticles to Brain Tumor Tae Jin Lee, Farzin Haque, Mario Vieweger, Ji Young Yoo, Balveen Kaur, Peixuan Guo, and Carlo M. Croce 11. Aptamer-Mediated Nanoparticle Interactions: From Oligonucleotide-Protein Complexes to SELEX Screens Laetitia Evade, Eric Dausse, Said Taouji, Emilie Daguerre, Eric Chevet, and Jean-Jacques Toulme 12. Methods for Assembling B-Cell Lymphoma Specific and Internalizing Aptamer-siRNA Nanoparticles Via the Sticky Bridge Jiehua Zhou, John J. Rossi, and Ka To Shum 13. A High Throughput Screening Assay for the Functional Delivery of Splice-Switching Oligonucleotides in Human Melanoma Cells John M. Dean and Robert K. DeLong 14. Design, Assembly, and Evaluation of RNA-Protein Nanostructures Hirohisa Ohno, Eriko Osada, and Hirohide Saito 15. Mapping RNA Interactions to Proteins in Virions Using CLIP-Seq Baochang Fan, Peng Ni, and C. Cheng Kao 16. Mapping Protein-RNA Interactions by RCAP, RNA-Crosslinking and Peptide Fingerprinting Robert Vaughan and C. Cheng Kao