Protein Amyloid Aggregation: Methods and Protocols (Paperback, Softcover Repri)

Part I: Labeling Strategies 1. Semisynthesis and Enzymatic Preparation of Post-Translationally Modified -Synuclein Bruno Fauvet and Hilal A. Lashuel 2. Isotope-Labeled Amyloids Via Synthesis, Expression, and Chemical Ligation for Use in FTIR, 2D IR, and NMR Studies Tianqi O. Zhang, Maksim Grechko, Sean D. Moran, and Martin T. Zanni Part II: Kinetics/Mechanism 3. Inter-Molecular Paramagnetic Relaxation Enhancement (PRE) Studies of Transient Complexes in Intrinsically Disordered Proteins Maria K. Janowska and Jean Baum 4. Detection of Helical Intermediates During Amyloid Formation by Intrinsically Disordered Polypeptides and Proteins Andisheh Abedini, Ping Cao, and Daniel P. Raleigh 5. Fluorescence Correlation Spectroscopy: A Tool to Study Protein Oligomerization and Aggregation In Vitro and In Vivo Bankanidhi Sahoo, Kenneth W. Drombosky, and Ronald Wetzel 6. Deep UV Resonance Raman Spectroscopy for Characterizing Amyloid Aggregation Joseph D. Handen and Igor K. Lednev 7. Analyzing Tau Aggregation with Electron Microscopy Carol J. Huseby and Jeff Kuret Part III: Oligomers 8. Characterization of Amyloid Oligomers by Electrospray Ionization-Ion Mobility Spectrometry-Mass Spectrometry (ESI-IMS-MS) Charlotte A. Scarff, Alison E. Ashcroft, and Sheena E. Radford 9. Formation and Characterization of -Synuclein Oligomers Wojciech Paslawski, Nikolai Lorenzen, and Daniel E. Otzen 10. Fluorescence Methods for Unraveling Oligomeric Amyloid Intermediates Niels Zijlstra, Nathalie Schilderink, and Vinod Subramaniam Part IV: Fibrils 11. Preparation of Amyloid Fibrils for Magic-Angle Spinning Solid-State NMR Spectroscopy Marcus D. Tuttle, Joseph M. Courtney, Alexander M. Barclay, and Chad M. Rienstra 12. Spin Labeling and Characterization of Tau Fibrils Using Electron Paramagnetic Resonance (EPR) Virginia Meyer and Martin Margittai 13. Preparation of Crystalline Samples of Amyloid Fibrils and Oligomers Asher Moshe, Meytal Landau, and David Eisenberg 14. Quenched Hydrogen Exchange NMR of Amyloid Fibrils Andrei T. Alexandrescu Part V: Computational Approaches 15. Studying the Early Stages of Protein Aggregation Using Replica Exchange Molecular Dynamics Simulations Joan-Emma Shea and Zachary A. Levine 16. Computational Methods for Structural and Functional Studies of Alzheimer's Amyloid Ion Channels Hyunbum Jang, Fernando Teran Arce, Joon Lee, Alan L. Gillman, Srinivasan Ramachandran, Bruce L. Kagan, Ratnesh Lal, and Ruth Nussinov 17. Analyzing Ensembles of Amyloid Proteins Using Bayesian Statistics Thomas Gurry, Charles K. Fisher, Molly Schmidt, and Collin M. Stultz Part VI: Toxicity and Pathology 18. In Vitro Studies of Membrane Permeability Induced by Amyloidogenic Polypeptides Using Large Unilamellar Vesicles Ping Cao and Daniel P. Raleigh 19. Cell Models to Study Cell-to-Cell Transmission of -Synuclein Eun-Jin Bae, He-Jin Lee, and Seung-Jae Lee 20. Preparation of Amyloid Fibrils Seeded from Brain and Meninges Kathryn P. Scherpelz, Jun-Xia Lu, Robert Tycko, and Stephen C. Meredith