Nuclear G-Protein Coupled Receptors: Methods and Protocols (Paperback, Softcover Repri)

1. Studies of Intracellular Angiotensin II Richard N. Re and Julia L. Cook 2. Single-Cell Microinjection Coupled to Confocal Microscopy to Characterize Nuclear Membrane Receptors in Freshly Isolated Cardiomyocytes Clemence Merlen and Jonathan Ledoux 3. Design and Application of Light-Activated Probes for Cellular Signaling David Chatenet, Steve Bourgault, and Alain Fournier 4. Using Caged Ligands to Study Intracrine Endothelin Signalling In Intact Cardiac Myocytes Clemence Merlen, Louis R. Villeneuve, and Bruce G. Allen 5. Quantification of Catecholamine Uptake in Adult Cardiac Myocytes Erika F. Dahl, Casey D. Wright, and Timothy D. O'Connell 6. Characterization of the Interaction Between the Prostaglandin D2 DP1 Receptor and the Intracellular L-Prostaglandin D Synthase Chantal Binda and Jean-Luc Parent 7. Isolation and Study of Cardiac Nuclei from Canine Myocardium and Adult Ventricular Myocytes Artavazd Tadevosyan, Bruce G. Allen, and Stanley Nattel 8. High Resolution Imaging and Function of Nuclear G Protein-Coupled Receptors (GPCRs) Vikrant K. Bhosle, Fernand Gobeil Jr., Jose Carlos Rivera, Alfredo Ribeiro-da-Silva, and Sylvain Chemtob 9. Biochemical Fractionation of Membrane Receptors in the Nucleus Ying-Nai Wang, Longfei Huo, Jennifer L. Hsu, and Mien-Chie Hung 10. Functional G Protein-Coupled Receptors on Nuclei From Brain and Primary Cultured Neurons Yuh-Jiin I. Jong and Karen L. O'Malley 11. Automated Microscopy of Cardiac Myocyte Hypertrophy: A Case Study on the Role of Intracellular a-Adrenergic Receptors Karen A. Ryall and Jeffrey J. Saucerman 12. Measuring Intra-Nuclear and Nuclear Envelope [Ca2+] vs. Cytosolic [Ca2+] Senka Ljuboyevic and Donald M. Bers 13. Assessing GPCR and G Protein Signaling to the Nucleus in Live Cells Using Fluorescent Biosensors Julie Bossuyt and Donald M. Bers 14. Tandem Affinity Purification to Identify Cytosolic and Nuclear Gbg-Interacting Proteins Rhiannon Campden, Darlaine Petrin, Melanie Robitaille, Nicolas Audet, Sarah Gora, Stephane Angers, and Terence E. Hebert 15. Examining the Effects of Nuclear GPCRs on Gene Expression Using Isolated Nuclei George Vaniotis, Sarah Gora, Andre Nantel, Terence E. Hebert, and Bruce G. Allen 16. Trafficking and Function of GPCRs in the Endosomal Compartment Davide Calebiro, Amod Godbole, Sandra Lyga, and Martin J. Lohse