Protein Microarrays: Methods and Protocols (Paperback, Softcover Repri)

Part I: Reverse Phase Protein Arrays 1. Reverse Phase Protein Microarrays for Clinical Applications Mariaelena Pierobon, Claudio Belluco, and Lance A. Liotta, and Emanuel F. Petricoin III 2. Impact of Blocking and Detection Chemistries on Antibody Performance for Reverse Phase Protein Arrays Kristi Ambroz 3. Phosphoprotein Stability in Clinical Tissue and Its Relevance for Reverse Phase Protein Microarray Technology Virginia Espina, Claudius Mueller, and Lance A. Liotta 4. Utilization of RNAi to Validate Antibodies for Reverse Phase Protein Arrays Heiko Mannsperger, Stefan Uhlmann, Ulrike Korf, and Ozgur Sahin 5. Antibody-Mediated Signal Amplification for Reverse Phase Protein Array-Based Protein Quantification Jan C. Brase, Heiko Mannsperger, Holger Sultmann, and Ulrike Korf 6. Reverse-Phase Protein Lysate Microarray (RPA) for the Experimental Validation of Quantitative Protein Network Models Satoshi Nishizuka 7. Characterization of Kinase Inhibitors Using Reverse Phase Protein Arrays Georg Martiny-Baron, Dorothea Haasen, Daniel D'Dorazio, Johannes Voshol, and Doriano Fabbro 8. Use of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues for Diagnosis and Therapy in Routine Clinical Settings Daniela Berg, Katharina Malinowsky, Bilge Ergin, Claudia Wolff, and Karl-Friedrich Becker 9. Producing Reverse Phase Protein Microarrays from Formalin Fixed Tissues Claudia Wolff, Claudia Schott, Katharina Malinowsky, Daniela Berg, and Karl-Friedrich Becker 10. Use of Reverse Phase Protein Microarrays to Study Protein Expression in Leukemia: Technical and Methodological Lessons Learned Steven M. Kornblau and Kevin C. Coombes Part II: Antibody Microarrays 11. Antibody Microarrays as Tools for Biomarker Discovery Marta Sanchez-Carbayo 12. Assessment of Antibody Specificity Using Suspension Bead Arrays Jochen M. Schwenk and Peter Nilsson 13. Quantitative Analysis of Phosphoproteins Using Microspot Immunoassays Frauke Henjes, Frank Gotschel, Anika Jocker, and Ulrike Korf 14. Robust Protein Profiling with Complex Antibody Microarrays in a Dual-Color Mode Christoph Schroder, Mohamed S.S. Alhamdani, Kurt Fellenberg, Andrea Bauer, Anette Jacob, and Jorg D. Hoheisel 15. High-Throughput Studies of Protein Glycoforms Using Antibody-Lectin Sandwich Arrays Brian B. Haab and Tingting Yue 16. Microspot Immunoassay-Based Analysis of Plasma Protein Profiles for Biomarker Discovery Strategies Johanna Sonntag, Heiko Mannsperger, Anika Joecker, and Ulrike Korf 17. Recombinant Antibodies for the Generation of Antibody Arrays Carl A.K. Borrebaeck and Christer Wingren Part III: Protein M icroarrays 18. Producing Protein Microarrays from DNA Microarrays Oda Stoevesandt, Michael J. Taussig, and Mingyue He 19. Cell Arrays and High-Content Screening Holger Erfle, Anastasia Eskova, Jurgen Reymann, and Vytaute Starkuviene 20. Probing Calmodulin Protein-Protein Interactions Using High Content Protein Arrays David J. O'Connell, Mikael Bauer, Sara Linse, and Dolores J. Cahill 21. Protein Function Microarrays for Customized Systems-Oriented Proteome Analysis Jonathan M. Blackburn and Aubrey Shoko 22. Optimized Autoantibody Profiling on Protein Arrays Sara L. O'Kane, John K. O'Brien, and Dolores J. Cahill Part IV: Sample Immobilization S trategies 23. Inkjet Printing for the Production of Protein Microarrays Iain McWilliam, Marisa Chong Kwan, and Duncan Hall 24. Impact of Substrates for Probe Immobilization Ursula Sauer 25. Contact Printing of Protein Microarrays John Austin