Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods (Paperback, Softcover Repri)

Part I. Screening and Design Purification Strategies 1. Protein Purification: An Overview Nikolaos Labrou 2. Establishment of a Design Space for Biopharmaceutical Purification Processes Using DoE Ignacio Amadeo, Laura Mauro, Eduardo Orti, and Guillermina Forno 3. High Throughput Process Development: I. Process Chromatography A. S. Rathore and R. Bhambure 4. High Throughput Process Development: II. Membrane Chromatography A. S. Rathore and S. Muthukumar 5. Media Selection in Ion Exchange Chromatography in a Single Microplate Charlotte Cabanne and Xavier Santarelli 6. High Throughput Screening of Dye-Ligands for Chromatography Sunil Kumar and Narayan S. Punekar 7. Measurement of Uptake Curves and Adsorption Isotherms by Automated Microscale Chromatography Pipette Tips Sunil Chhatre and Daniel G. Bracewell Part II. Low-Resolution Protein Purification Methods 8. Recovery of Recombinant Proteins from Plants Using Aqueous Two-Phase Partitioning Systems: An Outline Zhengrong GU 9. Aqueous Two-Phase Systems Strategies for the Recovery of Proteins from Plants Oscar Aguilar and Marco Rito-Palomares 10. Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic Proteins A. S. Rathore and R. Bhambure 11. Simultaneous Purification and Refolding of Proteins by Affinity Precipitation and Macro-(Affinity Ligand) Facilitated Three Phase Partitioning (MLFTPP) Ipsita Roy, Kalyani Mondal, and Munishwar N. Gupta 12. Co-Expression and Co-Purification of Antigen-Antibody Complexes in Bacterial Cytoplasm and Periplasm Ario de Marco 13. Immunoglobulin Purification by Caprylic Acid Victor Morais, Patricia Berasain, and Hugo Massaldi Part III: High-Resolution Protein Purification Methods 14. Affinity Tags in Protein Purification and Peptide Enrichment: An Overview Ana Sofia Pina, Iris L. Batalha, and Ana Cecilia A. Roque 15. Expression and Purification of Large Active GST Fusion Enzymes Stefania Deceglie, Claudia Lionetti, Marina Roberti, Palmiro Cantatore, and Paola Loguercio Polosa 16. Synthetic Ligand Affinity Chromatography. Purification of Human Serum Albumin and Related Fusion Proteins Sharon Williams, Phil Morton, and Dev Baines 17. Zbasic - A Purification Tag for Selective Ion-Exchange Recovery My Hedhammar, Johan Nilvebrant, and Sophia Hober 18. An Orthogonal Fusion Tag for Efficient Protein Purification Johan Nilvebrant, Mikael Astrand, and Sophia Hober 19. Phage Display of Engineered Binding Proteins Mark Levisson, Ruud B. Spruijt, Ingrid Nolla Winkel, Serve W.M. Kengen, and John van der Oost 20. Biomimetic Affinity Ligands for Protein Purification Isabel T. Sousa and M. Angela Taipa 21. Synthesis and Application of Dye-Ligand Affinity Adsorbents Evangelia Chronopoulou and Nikolaos E. Labrou 22. Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein Purification S. A. Camperi, M. C. Martinez-Ceron, S. L. Giudicessi, M. M. Marani, F. Albericio, and O. Cascone 23. Affinity Chromatography of Proteins on Monolithic Columns E.G. Vlakh, G.A. Platonova, and T.B. Tennikova 24. Sample Displacement Batch Chromatography of Proteins Marta Kotasinska, Verena Richter, Marcel Kwiatkowski, and Hartmut Schluter Part IV. Assessing Protein Structural Integrity, Purity, and Stabilization 25. Analysis of Host-Cell Proteins in Biotherapeutic Proteins by LC/MS Approaches Catalin E. Doneanu, Weibin Chen, and St John Skilton 26. Preparation of Monolithic Affinity Media for Nano-Liquid Chromatography Applications Jens Sproß and Andrea Sinz 27. Proteomic Analysis of Complex Protein Samples by MALDI TOF Mass Spectrometry Cosima Damiana Calvano, Cristina De Ceglie, and Carlo G. Zambonin 28. Modern Bioanalysis of Proteins by Electrophoretic Techniques Sona Krizkova, Marketa Ryvolova, Michal Masarik, O