Protein Chromatography: Methods and Protocols (Hardcover, 2, 2017)

Part I Overview Chapters 1. A Synopsis of Protein and their Purification Dermot Walls, Gary Cooney, and Sinead T. Loughran 2. Gel-Filtration Chromatography Ciaran O'Fagain, Philip M. Cummins, and Brendan F. O'Connor 3. Immunoaffinity Chromatography: Concepts and Applications Jenny Fitzgerald, Paul Leonard, Elaine Darcy, Shikha Sharma, and Richard O'Kennedy 4. Avoiding Proteolysis During Protein Purification Barry J. Ryan and Gary T. Henehan 5. Scale-Up of Protein Purification: Downstream Processing Issues John Joseph Milne 6. Phage Display: A Powerful Technology for the Generation of High Specificity Affinity Reagents from Alternative Immune Sources William J.J. Finlay, Laird Bloom, Joanne Grant, Edward Franklin, Deirdre Ni Shuilleabhain, and Orla Cunningham 7. Protein Stability: Enhancement and Measurement Ciaran O'Fagain 8. Tagging Recombinant Proteins to Enhance Solubility and Aid Purification Sinead T. Loughran and Dermot Walls Part II Protocols Chapters 9. Storage and Lyophilisation of Pure Proteins Ciaran O'Fagain and Keith Colliton 10. Differential Precipitation and Solubilization of Proteins Barry J. Ryan and Gemma K. Kinsella 11. Ion-Exchange Chromatography: Basic Principles and Application Philip M. Cummins, Keith D. Rochfort, and Brendan F. O'Connor 12. Protein Quantitation and Analysis of Purity Eva M. Campion, Sinead T. Loughran, and Dermot Walls 13. Purification of Proteins Fused to Maltose-Binding Protein Mario Lebendiker and Tsafi Danieli 14. Purification of Poly-Histidine-Tagged Proteins Sinead T. Loughran, Ronan T. Bree, and Dermot Walls 15. Purification of Antibodies Using Affinity Chromatography Elaine Darcy, Paul Leonard, Jenny Fitzgerald, Martin Danaher, Hui Ma, and Richard O' Kennedy 16. Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens William J.J. Finlay, Laird Bloom, Sreeja Varghese, Benedicte Autin, and Orla Cunningham 17. Measuring Protein-Protein Interactions Using Biacore Paul Leonard, Stephen Hearty, Hui Ma, and Richard O'Kennedy 18. Hydrophobic Interaction Chromatography Brendan F. O'Connor and Philip M. Cummins 19. Fast Protein Liquid Chromatography Ashkan Madadlou, Siobhan O'Sullivan, and David Sheehan 20. Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC/MS) Purification Systems Michael Henry and Paula Meleady 21. Strategies for the Purification of Membrane Proteins Sinead M. Smith 22. Antimicrobial Peptide Production and Purification Srinivas Suda, Des Field, and Niall Barron 23. Lectin Affiity Chromatography (LAC) ` Brendan F. O'Connor, Donal Monaghan, and Jonathan Cawley