Skeletal Muscle Development (Hardcover, 2017)

Part I: Skeletal Muscle Gene Expression 1. Bisulfite Sequencing for DNA Methylation Analysis of Primary Muscle Stem Cells Kohei Miyata, Masashi Naito, Tomoko Miyata, Sho Mokuda, and Hiroshi Asahara 2. Whole Genome Chromatin IP-Sequencing (ChIP-Seq) in Skeletal Muscle Cells Karl Kamhei So, Xianlu Peng, Hao Sun, and Huating Wang 3. Analysis of RNA Expression in Adult Zebrafish Skeletal Muscle Tamar E. Sztal, Peter D. Currie, and Robert J. Bryson-Richardson 4. Chick Somite Electroporation for Gene Function Studies Muhammad Abu-Elmagd Part II: Assays for Proliferating Skeletal Muscle Cells 5. Targeted Lipidomic Analysis of Myoblasts by GC-MS and LC-MS/MS Jordan Blondelle, Jean-Paul Pais de Barros, Fanny Pilot-Storck, and Laurent Tiret 6. Measuring Mitochondrial Substrate Utilization in Skeletal Muscle Stem Cells C. Hai Ly and James G. Ryall 7. Microcontact-Printed Hydrogel Microwell Arrays for Clonal Muscle Stem Cell Cultures Victor M. Aguilar and Benjamin D. Cosgrove 8. Isolation, Culture, and Differentiation of Fibro/Adipogenic Progenitors (FAPs) from Skeletal Muscle Robert N. Judson, Marcela Low, Christine Eisner, and Fabio M. Rossi 9. Human Satellite Cell Isolation and Xenotransplantation Steven M. Garcia, Stanley Tamaki, Xiaoti Xu, and Jason H. Pomerantz Part III: Assessment of Myogenic Differentiation 10. Application of Split-GFP Reassembly Assay to Study Myogenesis and Myofusion In Vitro Manami Kodaka, Xiaoyin Xu, Zeyu Yang, Junichi Maruyama, and Yutaka Hata 11. Myogenic Maturation by Optical-Training in Cultured Skeletal Muscle Cells Toshifumi Asano, Toru Ishizuka, and Hiromu Yawo 12. Fabrication of Micromolded Gelatin Hydrogels for Long-Term Culture of Aligned Skeletal Myotubes Gio C. Suh, Archana Bettadapur, Jeffrey W. Santoso, and Megan L. McCain Part IV: Assessing Skeletal Muscle Development In Vivo 13. Quantification of Embryonic Myofiber Development by Immunofluorescence Harika Nagandla and M. David Stewart 14. How to Wire the Diaphragm: Wholemount Staining Methods to Analyze Mammalian Respiratory Innervation Maximilian Michael Saller, Paolo Alberton, Andrea B. Huber, and Rosa Eva Huettl Part V: Muscle Repair Assays 15. Membrane Repair Assay for Human Skeletal Muscle Cells Romain Carmeille, Coralie Croissant, Flora Bouvet, and Anthony Bouter 16. Cryoinjury Model for Tissue Injury and Repair in Bioengineered Human Striated Muscle Richard J. Mills, Holly K. Voges, Enzo R. Porrello, and James E. Hudson