Breast Cancer: Methods and Protocols (Paperback, Softcover Repri)

Part I Detection of Molecular Markers of Breast Cancer 1. Basic Histopathological Methods and Breast Lesion Types for Research Nengtai Ouyang and Lin Wang 2. Clinical Applications for Immunohistochemistry of Breast Lesions Kester Haye, Rajarsi Gupta, Christopher Metter, and Jingxuan Liu 3. Immunohistochemistry for Triple-Negative Breast Cancer Kalnisha Naidoo and Sarah E. Pinder 4. In Situ Hybridization of Breast Cancer Markers Li Min and Chengchao Shou 5. Evaluation of Human Epidermal Growth Factor Receptor 2 (HER2) Gene Status in Human Breast Cancer Formalin-Fixed Paraffin Embedded (FFPE) Tissue Specimens by Fluorescence In Situ Hybridization (FISH) Harry C. Hwang and Allen M. Gown Part II Genetic Detection for Breast Cancer 6. Quantification of mRNA Levels Using Real-Time Polymerase Chain Reaction (PCR) Yiyi Li, Kai Wang, Longhua Chen, Xiaoxia Zhu, and Jie Zhou 7. Detection of MiRNA in Cultured Cells or Xenograft Tissues of Breast Cancer Martin Brown and Meiyun Fan 8. Pyrosequencing Analysis for Breast Cancer DNA Methylome Cem Kuscu and Canan Kuscu Part III Isolation of Breast Cancer Cells 9. Vita-Assay (TM) Method of Enrichment and Identification of Circulating Cancer Cells/Circulating Tumor Cells (CTCs) Shaun Tulley, Qiang Zhao, Huan Dong, Michael L. Pearl, and Wen-Tien Chen 1 0. Breast Cancer Stem Cell Isolation Xuanmao Jiao, Albert A. Rizvanov, Massimo Cristofanilli, Regina R. Miftakhova, and Richard G. Pestell Part IV In Vitro Experimental Models for Breast Cancer 11. Cellular Apoptosis Assay of Breast Cancer Yu Sun and Wei-Xing Zong 12. Assessment of Matrix Metalloproteinases by Gel Zymography Jillian Cathcart 13. Assessment of Synthetic Matrix Metalloproteinase Inhibitors by Fluorogenic Substrate Assay Ty J. Lively, Dale B. Bosco, Zahraa I. Khamis, and Qing-Xiang Amy Sang 14. Determination of Breast Cancer Cell Migratory Ability David Schmitt, Joel Andrews, and Ming Tan 15. A Novel Collagen Dot Assay for Monitoring Cancer Cell Migration Vincent M. Alford, Eric Roth, Qian Zhang, and Jian Cao 16. Three-Dimensional Assay for Studying Breast Cancer Cell Invasion Nikki A. Evensen 17. A Combined Phagocytosis and Fluorescent Substrate Degradation Assay to Simultaneously Assess Cell Migration and Substrate Degradation Ashleigh Pulkoski-Gross 18. Analysis of Invadopodia Formation in Breast Cancer Cells Ziqing Wang, Xiao Liang, Ming Cai, and Guangwei Du 19. Patient-Derived Tumor Xenograft Models of Breast Cancer Christopher D. Suarez and Laurie E. Littlepage 20. Monitoring Phosphatidic Acid Signaling in Breast Cancer Cells Using Genetically Encoded Biosensors Maryia Lu Li, Wei Rachel Tay, Jingquan He, and Guangwei Du 21. 3D In Vitro Model for Breast Cancer Research Using Magnetic Levitation and Bioprinting Method Fransisca Leonard and Biana Godin V In Vivo Experimental Models for Breast Cancer 22. Methods for Analyzing Tumor Angiogenesis in the Chick Chorioallantoic Membrane Model Jacquelyn J. Ames, Terry Henderson, Lucy Liaw, and Peter C. Brooks 23. Pharmacokinetics and Pharmacodynamics in Breast Cancer Animal Models Wei Wang, Subhasree Nag, and Ruiwen Zhang 24. Intracellular Delivery of Fluorescently Labeled Polysaccharide Nanoparticles to Cultured Breast Cancer Cells Derek Rammelkamp, Weiyi Li, and Yizhi Meng 25. Imaging Matrix Metalloproteinase Activity Implicated in Breast Cancer Progression Gregg B. Fields and Maciej J. Stawikowski