Protein Tyrosine Phosphatases: Methods and Protocols (Paperback, Softcover Repri)

1. The Extended Family of Protein Tyrosine Phosphatases Andres Alonso, Caroline E. Nunes-Xavier, Yolanda Bayon, and Rafael Pulido 2. Global RT-PCR and RT-qPCR Analysis of the mRNA Expression of the Human PTPome Caroline E. Nunes-Xavier and Rafael Pulido 3. Expression, Purification, and Kinetic Analysis of PTP Domains Mihaela Mentel, Rodica A. Badea, Georgiana Petrareanu, Sujay T. Mallikarjuna, Aura E. Ionescu, and Stefan E. Szedlacsek 4. Peptide Microarrays for Real Time Kinetic Profiling of Tyrosine Phosphatase Activity of Recombinant Phosphatases and Phosphatases in Lysates of Cells or Tissue Samples Liesbeth Hovestad-Bijl, Jeroen van Ameijde, Dirk Pijnenburg, Riet Hilhorst, Rob Liskamp, and Rob Ruijtenbeek 5. Tailor-Made Protein Tyrosine Phosphatases: In Vitro Site-Directed Mutagenesis of PTEN and PTPRZ-B Sandra Luna, Janire Mingo, Olaia Aurtenetxe, Lorena Blanco, Laura Amo, Jan Schepens, Wiljan J. Hendriks, and Rafael Pulido 6. Assays to Measure PTEN Lipid Phosphatase Activity In Vitro from Purified Enzyme or Immunoprecipitates Laura Spinelli and Nicholas R. Leslie 7. Assessing the Biological Activity of the Glucan Phosphatase Laforin Carlos Roma-Mateo, Madushi Raththagala, Mathew S. Gentry, and Pascual Sanz 8. Discovery and Evaluation of PRL Trimer Disruptors for Novel Anticancer Agents Yunpeng Bai, Zhi-Hong Yu, and Zhong-Yin Zhang 9. Analyzing Pseudophosphatase Function Shanta D. Hinton 10. Crystallization of PTP Domains Colin Levy, James Adams, and Lydia Tabernero 11. NMR Spectroscopy to Study MAP Kinase Binding to MAP Kinase Phosphatases Wolfgang Peti and Rebecca Page 12. Visualizing and Quantitating the Spatiotemporal Regulation of Ras/ERK Signaling by Dual-Specificity Mitogen-Activated Protein Phosphatases (MKPs) Christopher J. Caunt, Andrew M. Kidger, and Stephen M. Keyse 13. In Situ Proximity Ligation Assay (In Situ PLA) to Assess PTP-Protein Interactions Sina Koch, Irene Helbing, Sylvia-Annette Bohmer, Makoto Hayashi, Lena Claesson-Welsh, Ola Soderberg, and Frank-D. Bohmer 14. Use of Dominant Negative/Substrate Trapping PTP Mutations to Search for PTP Interactors/Substrates Vegesna Radha 15. Detection and Identification of Ligands for Mammalian RPTP Extracellular Domains Andrew William Stoker 16. Production of Osteoclasts for Studying Protein Tyrosine Phosphatase Signaling Eynat Finkelshtein, Einat Levy-Apter, and Ari Elson 17. Functional Analysis of Protein Tyrosine Phosphatases in Thrombosis and Haemostasis Souad Rahmouni, Alexandre Hego, Celine Delierneux, Odile Wera, Lucia Musumeci, Lutz Tautz, and Cecile Oury 18. Functional Analysis of Dual-Specificity Protein Phosphatases in Angiogenesis Mathieu Amand, Charlotte Erpicum, Christine Gilles, Agnes Noel, and Souad Rahmouni 19. Studying Protein-Tyrosine Phosphatases in Zebrafish Alexander James Hale and Jeroen den Hertog 20. Live staining of Drosophila Embryos with RPTP Fusion Proteins to Detect and Characterize Expression of Cell-Surface RPTP Ligands Namrata Bali, Hyung-Kook (Peter) Lee, and Kai Zinn 21. Methods to Study Protein Tyrosine Phosphatases Acting on Yeast MAPKs Almudena Sacristan-Reviriego, Maria Molina, and Humberto Martin