Fast Detection of DNA Damage: Methods and Protocols (Paperback, Softcover Repri)

Part I: EXPRESS DETECTION (from 3 minutes to 1 hour) 1. Express FRET Labeling and Analysis of Phagocytic Clearance Vladimir V. Didenko 2. Rapid Assessment of Genotoxicity by Flow Cytometric Detection of Cell Cycle Alterations Nevenka Bihari 3. Ultrasound Imaging of DNA-Damage Effects in Live Cultured Cells and in Brain Tissue Hadi Tadayyon, Mehrdad J. Gangeh, Roxana Vlad, Michael C. Kolios, and Gregory J. Czarnota 4. Ultrasound Imaging of Apoptosis: Spectroscopic Detection of DNA-Damage Effects In Vivo Hadi Tadayyon, Mehrdad J. Gangeh, Roxana Vlad, Michael C. Kolios, and Gregory J. Czarnota 5. Fluorochrome-Labeled Inhibitors of Caspases: Expedient In Vitro and In Vivo Markers of Apoptotic Cells for Rapid Cytometric Analysis Zbigniew Darzynkiewicz, Hong Zhao, H. Dorota Halicka, Piotr Pozarowski, and Brian Lee 6. The Fast-halo Assay for the Detection of DNA Aamage Piero Sestili, Cinzia Calcabrini, Anna Rita Diaz, Carmela Fimognari, and Vilberto Stocchi 7. Rapid Detection of Bacterial Susceptibility or Resistance to Quinolones Fatima Otero, Rebeca Santiso, Maria Tamayo, German Bou, Jaime Gosalvez, and Jose Luis Fernandez 8. Rapid Detection of Apoptosis in Cultured Mammalian Cells Kudryavtsev I., Serebryakova M., Solovjeva L., Svetlova M., and Firsanov D 9 Quick Detection of DNase II-type Breaks in Formalin-Fixed Tissue Sections Candace. L. Minchew and Vladimir V. Didenko Part II: RAPID DETECTION (3 hours or less) 10. Express Gamma-H2AX Immunocytochemical Detection of DNA Damage Nate Hopp, Jodi Hagen, Birte Aggeler, and Alexander E. Kalyuzhny 11. Rapid detection of Gamma-H2AX by Flow Cytometry in Cultured Mammalian Cells Firsanov D., Solovjeva L., Lublinskaya O., Zenin V., Kudryavtsev I., Serebryakova M., and Svetlova M. 12. Rapid Detection of DNA Strand Breaks in Apoptotic Cells by Flow- and Image- Cytometry Hong Zhao and Zbigniew Darzynkiewicz 13. Fast Micromethod: Determination of DNA Integrity in Cell Suspensions and in Solid Tissues Nevenka Bihari 14. Quantification of DNA Damage and Repair in Mitochondrial, Nuclear, and Bacterial Genomes by Real-Time PCR Nadja Patenge 15. Zebra Tail Amplification: Accelerated Detection of Apoptotic Blunt Ended DNA Breaks by In Situ Ligation Vladimir V. Didenko Part III: ACCELERATED DETECTION (time-saving versions of conventional techniques) 16. Twelve Gel Comet Assay Format for Quick Examination of DNA Damage and Repair Sergey Shaposhnikov and Andrew Collins 17. Immunofluorescence Analysis of Gamma-H2AX Foci in Mammalian Fibroblasts at Different Phases of the Cell Cycle Solovjeva L., Firsanov D., Pleskach N., and Svetlova M. 18. RAPD-PCR as a Rapid Approach for the Evaluation of Genotoxin-Induced Damage to Bacterial DNA Rosanna Tofalo and Aldo Corsetti 19. Rapid Detection of Gamma-H2Av Foci in ex vivo MMS-treated Drosophila Imaginal Discs Varandt Y. Khodaverdian and Mitch McVey