B Cell Receptor Signaling: Methods and Protocols (Paperback, Softcover Repri)

Preface... Table of Contents... Contributing Authors... 1. Activation-Induced Cytidine Deaminase Aided In Vitro Antibody Evolution Lili An, Chuan Chen, Ruiqi Luo, Yun Zhao, and Haiying Hang 2. Analyzing Mouse B-Cell Responses Specific to LCMV Infection Yaxing Hao, Zhirong Li, Yifei Wang, Xiaobing Liu, and Lilin Ye 3. Expression of Exogenous Genes in Murine Primary B Cells and B Cell Lines Using Retroviral Vectors Zhiyong Yang and Christopher D. C. Allen 4. Biophysical Techniques to Study B Cell Activation: Single Molecule Imaging and Force Measurements Ivan Rey, David A. Garcia, Brittany A. Wheatley, Wenxia Song, and Arpita Upadhyaya 5. DNA-Based Probes for Measuring Mechanical Forces in Cell-Cell Contacts: Application to B Cell Antigen Extraction from Immune Synapses Katelyn M. Spillane and Pavel Tolar 6. Deriving Quantitative Cell Biological Information from Dye-Dilution Lymphocyte Proliferation Experiments Koushik Roy, Maxim Nikolaievich Shokhirev, Simon Mitchell, and Alexander Hoffmann 7. Flow Cytometry Analysis of mTOR Signaling in Antigen-Specific B Cells Qizhao Huang, Haoqiang Wang, Lifan Xu, Jianjun Hu, Pengcheng Wang, Yiding Li, and Lilin Ye 8. Ex Vivo Culture Assay to Measure Human Follicular Helper T (Tfh) Cell-Mediated Human B Cell Proliferation and Differentiation Xin Gao, Lin Lin, and Di Yu ; 9. B Cell Receptor Signaling and Compartmentalization by Confocal Microscopy Anurag R. Mishral and Akanksha Chaturvedi 10. Investigate Early Activation Events of B Cell Receptor Signaling by Membrane Associated Antigen System and Total Internal Reflection Fluorescence Microscopy Xiaoming Bai, Xiaodong Zhao, and Chaohong Liu 11. Imaging the Interactions between B Cells and Antigen-Presenting Cellsal,Jia C. Wang, Madison Bolger-Munro, and Michael R. Gold 12. In Vivo Tracking of Particulate Antigen Localization and Recognition by B Lymphocytes at Lymph Nodes Yolanda R. Carrasco 13. Study B Cell Antigen Receptor Nano-scale Organization by In Situ Fab Proximity Ligation Assay Kathrin Klasener, Jianying Yang, and Michael Reth 14. Single Particle Tracking of Cell Surface Proteins Laabiah Wasim and Bebhinn Treanor 15. The Use of Intravital Two-Photon and Thick Section Confocal Imaging to Analyze B Lymphocyte Trafficking in Lymph Nodes and Spleen Chung Park, Il-Young Hwang, and John H. Kehrl 16. Time-lapse Forster Resonance Energy Transfer Imaging by Confocal Laser Scanning Microscopy for Analyzing Dynamic Molecular Interactions in the Plasma Membrane of B Cells Hae Won Sohn and Joseph Brzostowski 17. Understanding of B Cell Receptor Signaling through a Photo-Activatable Antigen Presentation System Jing Wang, Zhengpeng Wan, and Wanli Liur 18. Use of Streptolysin O-induced Membrane Damage as a Method of Studying the Function of Lipid Rafts during B Cell Activation Heather Miller and Wenxia Song 19. Visualization and Quantitative Analysis of the Actin Cytoskeleton upon B Cell Activation Vid Sustar, Marika Vainio, Pieta K. Mattila