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· 분류 : 외국도서 > 의학 > 유전학
· ISBN : 9781493985159
· 쪽수 : 227쪽
목차
1. 5'bromouridine IP Chase (BRIC)-Seq to Determine RNA Half Lives
??????????? Toshimichi Yamada, Naoto Imamachi, Rena Mizutani, Katsutoshi Imamura, Yutaka Suzuki, and Nobuyoshi Akimitsu
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2. Determining mRNA Decay Rates Using RNA Approach to Equilibrium Sequencing (RATE-Seq)
??????????? Farah Abdul-Rahman and David Gresham
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3. Metabolic Labeling of Newly Synthesized RNA with 4sU to in Parallel Assess RNA Transcription and Decay
??????????? Wei Sun and Wei Chen
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4. Measuring mRNA Decay in Budding Yeast Using Single Molecule FISH
??????????? Tatjana Trcek, Samir Rahman, and Daniel Zenklusen?
5. PAR-CLIP for Discovering Target Sites of RNA-Binding Proteins
??????????? Aitor Garzia, Pavel Morozov, Marcin Sajek, Cindy Meyer, and Thomas Tuschl
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6. Characterizing mRNA Sequence Motifs in the 3'UTR Using GFP Reporter Constructs
??????????? Rene Geissler and Andrew Grimson
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7. iCLIP of the PIWI Protein Aubergine in Drosophila Embryos
??????????? Bridlin Barckmann, Jeremy Dufourt, and Martine Simonelig
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8. Integration of ENCODE RNAseq and eCLIP Data Sets
??????????? Jorge Boucas
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9. Identifying miRNA Targets Using AgoRIP-Seq
??????????? Rebecca Petri and Johan Jakobsson
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10. Integrated Analysis of miRNA and mRNA Expression Profiles to Identify miRNA Targets
??????????? Zhiming Li and Chi-Meng Tzeng
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11. Identifying RISC Components Using Ago2 Immunoprecipitation and Mass Spectrometry
??????????? Tingfang Yi
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12. Using Tet-Off Cells and RNAi Knockdown to Assay mRNA Decay
??????????? Thomas D. Baird and J. Robert Hogg
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13. Identifying Cellular Nonsense-Mediated mRNA Decay (NMD) Targets: Immunoprecipitation of Phosphorylated UPF1 Followed by RNA Sequencing (p-UPF1 RIP-Seq)
??????????? Tatsuaki Kurosaki, Mainul Hoque, and Lynne E. Maquat
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14. Generation of Cell Lines Stably Expressing a Fluorescent Reporter of Nonsense-Mediated mRNA Decay Activity
??????????? Nadezhda M. Markina, Anton P. Pereverzev, Dmitry B. Staroverov, Konstantin A. Lukyanov, and Nadya G. Gurskaya
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15. Reactivation Assay to Identify Direct Targets of the Nonsense-Mediated mRNA Decay Pathway in Drosophila
??????????? Jonathan O. Nelson and Mark M. Metzstein
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16. Studying Nonsense-Mediated mRNA Decay in Mammalian Cells Using a Multicolored Bioluminescence-Based Reporter System
??????????? Andrew Nickless and Zhongsheng You
