Brain Tumor Stem Cells: Methods and Protocols (Hardcover, 2019)

1. Introduction to Brain Tumor Stem Cells Nicolas Yelle, David Bahkshinyan, Chitra Venugopal, and Sheila K. Singh 2. Isolation and Culture of Glioblastoma Brain Tumor Stem Cells Charles Chesnelong, Ian Restall, and Samuel Weiss 3. Establishment and Culture of Patient-Derived Primary Medulloblastoma Cell Lines Sara Badodi, Silvia Marino, and Loredana Guglielmi 4. Bioinformatic Strategies for the Genomic and Epigenomic Characterization of Brain Tumors Vijay Ramaswamy and Michael D. Taylor 5. Detecting Stem Cell Marker Expression Using the NanoString nCounter System Scott Ryall, Anthony Arnoldo, Javal Sheth, Sheila K. Singh, and Cynthia Hawkins 6. Flow Cytometric Analysis of Brain Tumor Stem Cells Minomi K. Subapanditha, Ashley A. Adile, Chitra Venugopal, and Sheila K. Singh 7. In Vitro Self-Renewal Assays for Brain Tumor Stem Cells Mathieu Seyfrid, David Bobrowski, David Bakhshinyan, Nazanin Tatari, Chitra Venugopal, and Sheila K. Singh 8. Differentiation of Brain Tumor Initiating Cells Michelle M. Kameda-Smith, Minomi K. Subapanditha, Sabra K. Salim, Chitra Venugopal, and Sheila K. Singh 9. The Study of Brain Tumor Stem Cell Migration Montserrat Lara-Velazquez, Rawan Al-kharboosh, Luis Prieto, Paula Schiapparelli, and Alfredo Quinones-Hinojosa 10. The Study of Brain Tumor Stem Cell Invasion Rawan Al-kharboosh, Montserrat Lara-Velazquez, Luis Prieto, Rachel Sarabia-Estrada, and Alfredo Quinones-Hinojosa 11. Cell Cycle Dynamics in Glioma Cancer Stem Cells Ingrid Qemo and Lisa A. Porter 12. Embryonic Stem Cell Models of Human Brain Tumors Ludivine Coudiere Morrison, Nazanin Tatari, and Tamra Werbowetski-Ogilvie 13. Chromatin Immunoprecipitation (ChIP) Protocols for the Cancer and Developmental Biology Laboratory Hunter McColl, Jamie L. Zagozewski, and David D. Eisenstat 14. EPH Profiling of BTIC Populations in Glioblastoma Multiforme Using CyTOF Amy Hu, Jarrett J. Adams, Parvez Vora, Maleeha Qazi, Sheila Singh, Jason Moffat, and Sachdev S. Sidhu 15. Pooled Lentiviral CRISPR-Cas9 Screens for Functional Genomics in Mammalian Cells Michael Aregger, Megha Chandrashekhar, Amy Tong, Kathrine Chan, and Jason Moffat 16. In Vitro Assays for Screening Small Molecules Ashley A. Adile, David Bakhshinyan, Chitra Venugopal, and Sheila K. Singh 17. Drug Delivery in an Orthotopic Tumor Stem Cell-Based Model of Human Glioblastoma Elena Binda, Alberto Visioli, Nadia Trivieri, and Angelo Luigi Vescovi 18. Engineering Inducible Knock-In Mice to Model Oncogenic Brain Tumor Mutations from Endogenous Loci Jon D. Larson and Suzanne J. Baker 19. In Vivo Murine Models of Brain Metastasis Mohini Singh, Neil Savage, and Sheila K. Singh 20. Cellular Magnetic Resonance Imaging for Tracking Metastatic Cancer Cells in the Brain Katie M. Parkins, Ashley V. Makela, Amanda M. Hamilton, and Paula J. Foster