G Protein-Coupled Receptor Signaling: Methods and Protocols (Hardcover, 2019)

Part I: Probing Structural Properties of GPCRs 1. Assessment of Conformational State Transitions of Class B GPCRs Using Molecular Dynamics Chenyi Liao, Victor May, and Jianing Li 2. Molecular Dynamic Simulations to Probe Water Permeation Pathways of GPCRs Katsufumi Tomobe, Eiji Yamamoto, and Kenji Yasuoka 3. Expression and Purification of a Functional E. coli 13 CH 3 -Methionine-Labeled Thermostable Neurotensin Receptor 1 Variant for Solution NMR Studies Fabian Bumbak, Ross A.D. Bathgate, Daniel J. Scott, and Paul R. Gooley 4. A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties Anne Zemella, Theresa Richter, Lena Thoring, and Stefan Kubick Part II: Tweaking Ligands for GPCR Studies 5. Furan Cross-Linking Technology for Investigating GPCR-Ligand Interactions Marleen Van Troys, Willem Vannecke, Christophe Ampe, and Annemieke Madder 6. Optical Regulation of Class C GPCRs by Photoswitchable Orthogonal Remotely-Tethered Ligands Amanda Acosta-Ruiz, Johannes Broichhagen, and Joshua Levitz 7. Chemoselective Acylation of Hydrazinopeptides to Access Fluorescent Probes for Time-Resolved FRET Assay on GPCRs Sridevi M. Ramanoudjame, Lucie Esteoulle, Stephanie Riche, Jean-Francois Margathe, Thierry Durroux, Iuliia A. Karpenko, and Dominique Bonnet Part III: Bioluminescence and Fluorescence Resonance Transfer Energy Approaches 8. Time-Resolved FRET-Based Assays to Characterize G Protein-Coupled Receptor Hetero-Oligomer Pharmacology Joyce Heuninck, Candide Hounsou, Elodie Dupuis, Eric Trinquet, Bernard Mouillac, Jean-Philippe Pin, Dominique Bonnet, and Thierry Durroux 9. Combining Conformational Profiling of GPCRs with CRISPR/Cas9 Gene Editing Approaches Kyla Bourque, Dominic Devost, Asuka Inoue, and Terence E. Hebert 10. Measuring GPCR Stoichiometry Using Types -1, -2 and -3 Bioluminescence Resonance Energy Transfer-Based Assays James H. Felce, John R. James, and Simon J. Davis 11. Combining SRET 2 and BiFC to Study GPCR Heteromerization and Protein-Protein Interactions Amina M. Bagher, Melanie E.M. Kelly, and Eileen M. Denovan-Wright 12. Quantification and Comparison of Signals Generated by Different FRET-Based cAMP Reporters Andreas Koschinski and Manuela Zaccolo Part IV: Exploring GPCR Signaling Properties 13. Measuring GPCR-Induced Activation of Protein Tyrosine Phosphatases (PTP) Using In-Gel and Colorimetric PTP Assays Genevieve Hamel-Cote, Fanny Lapointe, and Jana Stankova 14. Measurement of beta-Arrestin Recruitment at GPCRs Using the Tango Assay Genevieve Laroche and Patrick M. Giguere 15. Probing the Interactome of Corticotropin-Releasing Factor Receptor Heteromers Using Mass Spectrometry Burcu Hasdemir, Juan A. Oses-Prieto, Alma Burlingame, and Aditi Bhargava Part V: Visualizing Subcellular Compartmentalization of GPCRs 16. Monitoring the Aggregation of GPCRs by Fluorescence Microscopy Samuel Genier, Jade Degrandmaison, Christine L. Lavoie, Louis Gendron, and Jean-Luc Parent 17. Combining RNAi and Immunofluorescence Approaches to Investigate Post-Endocytic Sorting of GPCRs into Multivesicular Bodies Xuezhi Li, Stephanie Rosciglione, Andreanne Laniel, and Christine Lavoie 18. Super-Resolution Imaging of G Protein-Coupled Receptors Using Ground State Depletion Microscopy Fabiana A. Cateano-Crowley, Bryan Heit, and Stephen S.G. Ferguson 19. Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy Kim C. Jonas and Aylin C. Hanyaloglu Part VI: Investigating In Vivo Functions of GPCRs 20. Optical Modulation of Metabotropic Glutamate Receptor Type 5 In Vivo Using a Photoactive Drug Marc Lopez-Cano, Joan Font, Amadeu Llebaria, Victor Fernandez-Duenas, and Francisco Ciruela 21. Imaging of Tissue-Specific and Temporal Activation of GPCR Sign