Lymphoma: Methods and Protocols (Hardcover, 2, 2019)

Preface... Table of Contents... Contributing Authors... 1. Origin and Pathogenesis of B Cell Lymphomas Marc Seifert, Rene Scholtysik, and Ralf Kuppers 2. Flow Cytometry for Non-Hodgkin and Hodgkin Lymphoma Emily Glynn, Lori Soma, David Wu, Brent L. Wood, and Jonathan R. Fromm 3. Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B Cells Ralf Kuppers, Markus Schneider, and Martin-Leo Hansmann 4. PCR GeneScan and Heteroduplex Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes for Clonality Diagnostics in Suspect Lymphoproliferations Elke Boone, Kim C. Heezen, Patricia JTA Groenen, and Anton W. Langerak 5. Expression Cloning of Antibodies from Single Human B Cells Hedda Wardemann and Christian E. Busse 6. Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR Menno C. van Zelm 7. Stereotyped B Cell Immunoglobulins in B Cell Lymphomas Andreas Agathangelidis, Fotis Psomopoulos, and Kostas Stamatopoulos 8. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies Sebastian Bottcher 9. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR Christiane Pott, Monika Bruggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba 10. Generation of Whole Genome Bisulfite Sequencing Libraries from Very Low DNA Input Dieter Weichenhan, Charles D. Imbusch, Qi Wang, Benedikt Brors, and Christoph Plass 11. FISH and FICTION in Lymphoma Research Maciej Giefing and Reiner Siebert 12. NGS-Based High-Throughput Screen to Identify MicroRNAs Regulating Growth of B-Cell Lymphoma Joost Kluiver, Fubiao Niu, Ye Yuan, Klaas Kok, Anke van den Berg, and Agnieszka Dzikiewicz-Krawczyk 13. RNA Sequencing in B-Cell Lymphomas Da Wei Huang, Moez Dawood, Calvin A. Johnson, and Roland Schmitz 14. Studying Cancer Heterogeneity by Single Cell RNA-Sequencing Johannes W. Bagnoli, Lucas E. Wange, Aleksandar Janjic, and Wolfgang Enard 15. Evaluation of Whole Genome Sequencing Data Daniel Huebschmann and Matthias Schlesner 16. Protocols for CRISPR-Cas9 Screening in Lymphoma Cell Lines Daniel E. Webster, Sandrine Roulland, and James Phelan 17. Lymphoma and Leukemia Cell Vulnerabilities and Resistance Identified by Compound Library Screens Katarzyna Tomska, Sebastian Scheinost, and Thorsten Zenz 18. Proximity Ligation Assay Ryan M. Young 19. Phospoproteomic Analysis of Signaling Pathways in Lymphomas Bjorn Haupl, Henning Urlaub, and Thomas Oellerich 20. Ultrasensitive Detection of Circulating Tumour DNA in Lymphoma via Targeted Hybridization Capture and Deep Sequencing of Barcoded Libraries Miguel Alcaide, Christopher Rushton, and Ryan D. Morin