Molecular Cardiology: Methods and Protocols (Hardcover, 2005)

Part I. Introduction Introduction to Methods in Molecular Cardiology Zhongjie Sun Part II. Gene Transfer and Gene Therapy for Cardiovascular Disease Gene Therapy Approaches to Cardiovascular Disease J. Mark Jones and Walter J. Koch Gene Therapy for Myocardial Ischemia Using the Hypoxia-Inducible Double Plasmid System Yi Tang Cardiac Gene Delivery Using DNA-Adenoviral Constructs J. Mark Jones and Walter J. Koch Manipulation of Neuronal Nitric Oxide Synthase Within the Paraventricular Nucleus Using Adenovirus and Antisense Technology Yi-Fan Li, Yu Wang, Keith M. Channon, Harold D. Schultz, Irving H. Zucker, and Kaushik P. Patel TAT-Mediated Protein Transduction: Delivering Biologically Active Proteins to the Heart Asa B. Gustafsson, Roberta A. Gottlieb, and David J. Granville Expression of Human eNOS in Cardiac and Endothelial Cells Xiuqing Wang, Robert Cade, and Zhongjie Sun Strategies of Conditional Gene Expression in Myocardium: An Overview Heather L. Heine, Hon Sing Leong, Fabio M. V. Rossi, Bruce M. McManus, and Thomas J. Podor Gene Silencing Using Adenoviral RNAi Vector in Vascular Smooth Muscule Cells and Cardiomyocytes Hideko Kasahara and Hiroki Aoki Part III. Stem Cell Therapy for Cardiovascular Disease Cardiac Commitment of Embryonic Stem Cells for Myocardial Repair Dana Zeineddine, Evangelia Papadimou, Annabelle Mery, Claudine Menard, and Michel Puceat Autologous Mesenchymal Stem Cells for Post-Ischemic Myocardial Repair Yao Liang Tang Cellular Therapy With Autologous Skeletal Myoblasts for Ischemic Heart Disease and Heart Failure Yao Liang Tang Protocols for Myocardial Infarction Repair Using Fetal Cardiac Myocytes Sharon Etzion, Radka Holbova, Liron Miller, and Jonathan Leor Methods for Examining Stem Cells inPost-Ischemic and Transplanted Hearts Nana Rezai, Hubert Walinski, Alexandra Kerjner, Lubos Bohunek, Fabio M. V. Rossi, Bruce M. McManus, and Thomas J. Podor Endothelial Progenitor Cell Culture and Gene Transfer Hideki Iwaguro and Takayuki Asahara Part IV. Gene Analysis in the Injured and Hypertrophied Heart Delineation of Sequences Essential for Specific Promoter Activation During Pressure Overloaded Hypertrophy or Factor-Induced Hypertrophy Chellam Rajamanickam and Radhakrishnan Jeejabai. Evaluation of the Cardiac Isoform of a2-Macroglobulin as a Factor Inducing Cardiac Hypertrophy Chellam Rajamanickam and Radhakrishnan Jeejabai Detection of Apoptosis in Cardiovascular Diseases Arwen L. Hunter, Jonathan C. Choy, and David J. Granville Detection of Cardiac Signaling in the Injured and Hypertrophied Heart Xiaoning Si, Maziar Rahmani, Ji Yuan, and Honglin Luo Affymetrix Oligonucleotide Analysis of Gene Expression in the Injured Heart Bobby Yanagawa, Lydia Taylor, Theresa A. Deisher, Raymond Ng, George F. Schreiner, Timothy J. Triche, Decheng Yang, and Bruce M. McManus Identification and Validation of Loci and Candidate Genes Linked to Cardiac Hypertrophy Bastien Llamas and Christian F. Deschepper Induction and Analysis of Cardiac Hypertrophy in Transgenic Animal Models Marcos E. Barbosa, Natalia Alenina, and Michael Bader Methods for the Detection of Altered b-Adrenergic Receptor Signaling Pathways in Hypertrophied Hearts Matthew J. Wolf, Hideo Tachibana, and Howard A. Rockman Part V. Transgenes in Cardiovascular Research Transgenic Mouse Models for Cardiac Dysfunction by a Specific Gene Manipulation Gopal J. Babu and Muthu Periasamy Cardiomyocyte Defects in Diabetic Models and Protection With Cardiac-Targeted Transgenes Xia Shen, Gang Ye, Naira S. Metreveli, and Pau