Plant Virology Protocols: From Viral Sequence to Protein Function (Hardcover, 2)

Section 1 General Introduction 1. Plant virus interactions -Peter Palukaitis, John Carr and James Schoelz (EJ) 2. Role of capsid proteins - John Bol (EJ) 3. Role of plant virus movement proteins - Michael Taliansky, Lesley Torrance, Natalia O. Kalinina (EJ) 4. Multiple roles of viral replication proteins in plant RNA virus replication- Peter Nagy and Judith Pogany (PN) 5. Role of silencing suppressor proteins -Jozsef Burgyan (EJ) 6. Role of vector transmission proteins - Veronique Ziegler Graff, Veronique Brault (EJ) Section 2 Viral nucleic acid sequence/function analysis 7. In vivo analysis of Viral RNA translation- William Staplin, Allen Miller (PN) 8. In vitro analysis of translational enhancer -Aurelie Rakotondrafara, Allen Miller (YH) 9. Identification of plant virus IRES - Sek-Man Wong, Dora Chin-Yen Koh, DingXiang Liu (YH) 10. Analysis of geminivirus DNA replication by 2-D gel - Keith Saunders (YH) 11. Begomoviruses: Molecular cloning and identification of replication origin - Lilian H. Florentino, Anesia A. Santos, Francisco M. Zerbini, Elizabeth P. B. Fontes (YH) 12. Analysis of viroid replication - Ricardo Flores, Maria-Eugenia Gas, Diego Molina, Carmen Hernandez, and Jose-Antonio Daros (PN) 13. Biochemical Analyses of the Interactions Between Viral Polymerases and RNAs- Young-Chan Kim and C. Cheng Kao (PN) 14. In situ detection of plant viruses and virus-specific products - Andy Maule and Zoltan Havelda (PN) 15. Detection of siRNAs and miRNAs- Sakari Kauppinen, Zoltan Havelda (EJ) 16. Cloning of short interfering RNAs from virus infected plants - Thien X. Ho, Rachel Rusholme, Tamas Dalmay, and Hui Wang (YH) 17. Solution structure probing of RNA structures - Marc R. Fabian and K. Andrew White (PN) 18. RNA encapsidation assay - Padmanaban Annamalaiand A. L. N. Rao (GF) Section 3 Protein analysis and investigation of protein function 19. Surface plasmon resonance analysis of interactions between replicase proteins of tomato bushy stunt virus- KS. Rajendran and Peter Nagy (GF) 20. Biochemical approaches for characterizing RNA-protein complexes in preparation for high resolution structure analysis- Raul C. Gomila and Lee Gehrke (PN) 21. Probing interactions between plant virus movement proteins and nucleic acids - Tzvi Tzfira, Vitaly Citovski (PN) 22. Movement profiles, a tool for quantitative analysis of cell-to-cell movement of plant viral movement proteins - Kateryna Trutnyeva, Pia Ruggenthaler, Elisabeth Waigmann (PN) 23. Analysis of siRNA-supressor of gene silencing interactions - Lorant Lakatos and Jozsef Burgyan (EJ) 24. Phosphorylation analysis of plant virus proteins - Kristiina M. Makinen and Konstantin I. Ivanov (PN) 25. Analysis of interactions between viral replicase proteins and plant intracellular membranes- Helene Sanfacon and Guangzhi Zhang (PN) 26. Membrane and protein dynamics in virus-infected plant cells - Michael Goodin, Romit Chakrabarty, Sharon Yelton (PN) 27. Site-directed mutagenesis of whole viral genomes - Li liu, George Lomonossoff (YH) 28. Viral protein-nucleic acid interaction: South(North)-Western blot - Huanting Liu (YH) 29. Protein-protein interactions: yeast two hybrid system - Deyin Guo, Minna-Liisa Rajamaki, and Jari Valkonen (YH) 30. NMR analysis of viral protein structures - Andrew J. Dingley, Inken Lorenzen and Joachim Grotzinger (YH) 31. Localization of viral protein in plant cells: protein tagging -Sophie Haupt, Lesley Torrance (GF) Section 4 Microscopy/GFP/Protein tagging/infections clones and other such tools 32. Construction of infectious clones for RNA viruses -