Prion Protein Protocols (Hardcover, 2008)

Chapter 1 Cell culture models to unravel prion protein function and aberrancies in prion diseases Katarina Bedecs Chapter 2 Investigation of PrPC metabolism and function in live cells: methods for studying individual cells and cell populations. Cathryn L. Haigh, David R. Brown Chapter 3 Immunodetection of PrPSc using Western and slot blotting techniques Hanna Gyllberg and Kajsa Lofgren Chapter 4 Assaying Prions in Cell Culture: The Standard Scrapie Cell Assay (SSCA) and the Scrapie Cell Assay in End Point Format (SCEPA) Sukhvir P. Mahal, Cheryl A. Demczyk, Emery W. Smith Jr., Peter-Christian Klohn and Charles Weissmann Chapter 5 Generation of cell lines propagating infectious prions and the isolation and characterization of cell derived exosomes. Laura J Vella and Andrew F Hill. Chapter 6 Neurotoxicity of prion peptides on cultured cerebellar neurons Giuseppe D. Ciccotosto, Roberto Cappai and Anthony R. White Chapter 7 Understanding the nature of prion diseases using cell-free assays. Victoria A. Lawson Chapter 8 Methods for Conversion of Prion Protein into Amyloid Fibrils. Leonid Breydo, Natallia Makarava and Ilia V. Baskakov Chapter 9 Amplification of Purified Prions In Vitro Surachai Supattapone, Nathan R. Deleault, and Judy R. Rees Chapter 10 Expression and Purification of Full-Length Recombinant PrP of High Purity Natallia Makarava and Ilia V. Baskakov Chapter 11 Analysis of PrP conformation using circular dichroism Sen Han, and Andrew F. Hill Chapter 12 Effect of copper on the de novo generation of prion protein expressed in Pichia pastoris CarinaTreiber Chapter 13 Biophysical Investigations of the prion protein using Electron Paramagnetic Resonance Simon C. Drew and Kevin J. Barnham Chapter 14 Molecular diagnosis of human prion disease Jonathan D.F. Wadsworth, Caroline Powell, Jonathan A. Beck, Susan Joiner, Jacqueline M. Linehan, Sebastian Brandner, Simon Mead and John Collinge Chapter 15 Analysis of endogenous PrPC processing in neuronal and non-neuronal cell lines. Victoria Lewis and Steven J Collins Chapter 16 Molecular typing of PrPres in human sporadic CJD brain tissue. Victoria Lewis, Genevieve M Klug, Andrew F Hill and Steven J Collins Chapter 17 Transgenic mouse models of prion diseases Glenn Telling Chapter 18 Quantitative bioassay of surface bound prion infectivity. Victoria A. Lawson