Antibody Phage Display: Methods and Protocols (Hardcover, 2, 2009)

Table of Contents Preface Contributors Antibody phage display - overview of a powerful technology that has quickly translated to the clinic Beatrix Kotlan and Mark C. Glassy Design and construction of synthetic phage-displayed Fab libraries Jenny Bostrom and Germaine Fuh Affinity isolation of antigen-specific circulating B cells for generation of phage display-derived human monoclonal antibodies Henrik J. Ditzel Construction of phage antibody repertoires from the blood of West Nile virus infected donors Mark Throsby and John de Kruif Anti-ß2GP-I and anti-prothrombin antibodies generated by phage display Javier Cabiedes and Antonio R. Cabral The generation and selection of single-domain, V region libraries from nurse sharks Martin F. Flajnik and Helen Dooley The isolation of scFvs against small target molecules Iain Shaw and Marian Kane Display and selection of scFv antibodies on HEK-293T cells Mitchell Ho and Ira Pastan Isolation of scFvs that inhibit the NS3 protease of Hepatitis C virus by a combination of phage display and a bacterial genetic screen Limor Nahary, Alla Trahtenherts and Itai Benhar Guided selection methods through chain shuffling Bao Guo-qiang and He Xian-Li Sequential antigen panning for selection of broadly cross-reactive HIV-1 neutralizing human monoclonal antibodies Mei-Yun Zhang and Dimiter S. Dimitrov Selection of antibodies able to rapidly enter mammalian cells Marie-Alix Poul Identification of the specificity of isolated phage display single chain antibodies using yeast 2-hybrid screens Nicolaj Rasmussen and Henrik J.Ditzel Generation of bispecific and tandem diabodies Sergey M. Kipriyanov Intrabody expression in eukaryotic cellsLaurence Guglielmi and Pierre Martineau High level periplasmic expression and purification of scFvs Sergey M. Kipriyanov Expression of single-chain Fv fragments in E. coli cytoplasm Laurence Guglielmi and Pierre Martineau High-level expression of a phage display-derived scFv in Pichia pastoris Leonardo M. Damasceno, Frank Lee, Gerd Ritter, Lloyd Old, and Carl Batt