Histology Protocols (Hardcover)

Part I: Tissue Processing 1. Tissue Preparation for Histochemistry: Fixation, Embedding, and Antigen Retrieval for Light Microscopy Tim D. Hewitson, Belinda Wigg, and Gavin J. Becker 2. An Optimized RNA Extraction Method from Archival Formalin Fixed-Paraffin Embedded Tissue Joon-Yong Chung and Stephen M. Hewitt 3. Laser Capture Microdissection and Pressure-Catapulting for the Analysis of Gene Expression in the Renal Glomerulus Amanda J. Edgley, Renae M. Gow, and Darren J. Kelly Part II: Staining Techniques 4. Immunofluorescence Detection of the Cytoskeleton and Extracellular Matrix in Tissue and Cultured Cells Josiane Smith-Clerc and Boris Hinz 5. Double Immunohistochemistry with Horseradish Peroxidase and Alkaline Phosphatase Detection Systems Vincent Sarrazy and Alexis Desmouliere 6. Retrogradely-Transported Neuronal Tracers Combined with Immunohistochemistry Using Free Floating Brain Sections Emilio Badoer 7. High-Pressure Freezing, Chemical Fixation, and Freeze-Substitution for Immuno-Electron Microscopy Christian Muhlfeld 8. Lectin Histochemistry for Light and Electron Microscopy Su Ee Wong, Catherine Winbanks, Chrishan S. Samuel, and Tim D. Hewitson 9. Duplex In Situ Hybridization in the Study of Gene Co-Regulation in the Vertebrate Brain Raphael Pinaud and Jin K. Jeong 10. Special Stains for Extracellular Matrix Andrea Monte-Alto-Costa and Luis Cristovao de Moraes Sobrino Porto 11. Active Staining of Mouse Embryos for Magnetic Resonance Microscopy Alexandra Petiet and G. Allan Johnson 12. Immunohistochemical Detection of Tumor Hypoxia Richard J. Young and Andreas Moller 13. In Situ Localization of Apoptosis Using TUNEL Tim D. Hewitson and Ian A.Darby Part III: Imaging Techniques 14. Use of Confocal Microscopy for 3-Dimensional Imaging of Neurons in the Spinal Cord Martin Stebbing, Simon Potocnik, Pinglu Ye, and Emilio Badoer 15. High Resolution Confocal Imaging in Tissue Verena C. Wimmer and Andreas Moller 16. Software-Based Stacking Techniques to Enhance Depth of Field and Dynamic Range in Digital Photomicrography Jorg Piper 17. Image Analysis and Quantitative Morphology Carlos Alberto Mandarim-de-Lacerda, Caroline Fernandes-Santos, and Marcia Barbosa Aguila