Cartilage and Osteoarthritis (Paperback)

Culture and Phenotyping of Chondrocytes in Primary Culture Sylvie Thirion and Francis Berenbaum Culture of Chondrocytes in Alginate Beads Frederic De Ceuninck, Christophe Lesur, Philippe Pastoureau, Audrey Caliez, and Massimo Sabatini Immortalization of Human Articular Chondrocytes for Generation of Stable, Differentiated Cell Lines Mary B. Goldring Culture of Immortalized Chondrocytes and Their Use As Models of Chondrocyte Function Mary B. Goldring Generation of Pluripotent Stem Cells and Their Differentiation to the Chondrocytic Phenotype Luis A. Solchaga, Jean F. Welter, Donald P. Lennon, and Arnold I. Caplan Semiquantitative Analysis of Gene Expression in Cultured Chondrocytes by RT-PCR Gaelle Rolland-Valognes Quantification of mRNA Expression Levels in Articular Chondrocytes With PCR Technologies Audrey McAlinden, Jochen Haag, Brigitte Bau, Pia M. Gebhard, and Thomas Aigner RNA Extraction From Cartilage Frederic Mallein-Gerin and Jerome Gouttenoire Gene Expression Analysis in Cartilage by In Situ Hybridization Frederic Mallein-Gerin and Jerome Gouttenoire Analysis of Differential Gene Expression in Healthy and Osteoarthritic Cartilage and Isolated Chondrocytes by Microarray Analysis Thomas Aigner, Joachim Saas, Alexander Zien, Ralf Zimmer, Pia M. Gebhard, and Thomas Knorr High-Efficiency Nonviral Transfection of Primary Chondrocytes Jean F. Welter, Luis A. Solchaga, and Matthew C. Stewart In Vitro Gene Transfer to Chondrocytes and Synovial Fibroblasts by Adenoviral Vectors Jean-Noel Gouze, Martin J. Stoddart, Elvire Gouze, Glyn D. Palmer, Steven C. Ghivizzani, Alan J. Grodzinsky, and Christopher H. Evans Changes of Chondrocyte Metabolism In Vitro: An Approach by Proteomic Analysis Anne-Marie Freyria and Michel Becchi Analysis of ChondrocyteFunctional Markers and Pericellular Matrix Components by Flow Cytometry Gust Verbruggen, Jun Wang, Lai Wang, Dirk Elewaut, and Eric M. Veys A Simple and Reliable Assay of Proteoglycan Synthesis by Cultured Chondrocytes Frederic De Ceuninck and Audrey Caliez Assays of Proteoglycan and Collagen Degradation in Cultures of Rabbit Cartilage Explants Christophe Lesur and Massimo Sabatini Production of Antibodies Against Degradative Neoepitopes in Aggrecan John S. Mort and Peter J. Roughley Immunoassays for Collagens in Chondrocyte and Cartilage Explant Cultures R. Clark Billinghurst, Fackson Mwale, Anthony Hollander, Mirela Ionescu, and A. Robin Poole Detection of Apoptosis in Cartilage In Situ and in Isolated Chondrocytes Darryl D. D'Lima, Klaus Kuhn, and Martin K. Lotz Expression, Activity, and Regulation of MAP Kinases in Cultured Chondrocytes Jang-Soo Chun Mechanical Loading of Chondrocytes Embedded in 3D Constructs: In Vitro Methods for Assessment of Morphological and Metabolic Response to Compressive Strain David A. Lee and Martin M. Knight In Vitro Physical Stimulation of Tissue-Engineered and Native Cartilage Kelvin W. Li, Travis J. Klein, Kanika Chawla, Gayle E. Nugent, Won C. Bae, and Robert L. Sah Index