Cystic Fibrosis: Diagnosis and Protocols, Volume 2: Methods and Resources to Understand Cystic Fibrosis (Hardcover)

Part I: Pathophysiology of Cystic Fibrosis 1. Introduction to Part I: Overview of Approaches to Study Cystic Fibrosis Pathophysiology Mark T. Clunes and Richard C. Boucher 2. Imaging CFTR Protein Localization in Cultured Cells and Tissues Silvia M. Kreda and Martina Gentzsch 3. CFTR Regulation of Epithelial Sodium Channel Yawar J. Qadri, Estelle Cormet-Boyaka, Dale J. Benos, and Bakhrom K. Berdiev 4. Methods for Evaluating Inflammation in Cystic Fibrosis Assem G. Ziady and Pamela B. Davis 5. Methods for ASL Measurements and Mucus Transport Rates in Cell Cultures Erin N. Worthington and Robert Tarran 6. Measurement of Fluid Secretion from Intact Airway Submucosal Glands Jeffrey J. Wine, Nam Soo Joo, Jae Young Choi, Hyungju Cho, Mauri E. Krouse, Jin V. Wu, Monal Khansaheb, Toshiya Irokawa, Juan Ianowski, John W. Hanrahan, Alan W. Cuthbert, and Kim V. Tran 7. Measurements of Intracellular Calcium Signals in Polarized Primary Cultures of Normal and Cystic Fibrosis Human Airway Epithelia Carla M.P. Ribeiro 8. Identification and Quantification of Mucin Expression Kristina A. Thomsson and Gunnar C. Hansson 9. Methods to Classify Bacterial Pathogens in Cystic Fibrosis Thomas Bjarnsholt, Xiaohui Chen Nielsen, Ulla Johansen, Lena Nørgaard, and Niels Høiby 10. Approaches to Study Differentiation and Repair of Human Airway Epithelial Cells Sophie Crespin, Marc Bacchetta, Song Huang, Tecla Dudez, Ludovic Wiszniewski, and Marc Chanson Part II: Omic Approaches to Study Cystic Fibrosis 11. Introduction to Part II: Omics in the Biology of Cystic Fibrosis William E. Balch 12. Microarray mRNA Expression Profiling to Study Cystic Fibrosis Shyam Ramachandran, Luka A. Clarke, Todd E. Scheetz, Margarida D. Amaral, and Paul B. McCray, Jr. 13. Quantitative Differential Proteomics of Cystic Fibrosis Cell Models by SILAC (Stable Isotope Labelling in Cell Culture) Ida Chiara Guerrera, Mario Ollero, Diane-Lore Vieu, and Aleksander Edelman 14. Application of Mass Spectrometry to Study Proteomics and Interactomics in Cystic Fibrosis William E. Balch and John R. Yates III 15. Functional Genomics Assays to Study CFTR Traffic and ENaC Function Joana Almaca, Shehrazade Dahimene, Nicole Appel, Christian Conrad, Karl Kunzelmann, Rainer Pepperkok, and Margarida D. Amaral 16. New Lipidomic Approaches in Cystic Fibrosis Mario Ollero, Ida Chiara Guerrera, Giuseppe Astarita, Daniele Piomelli, and Aleksander Edelman Part III: Resources 17. Introduction to Part III: Resources for CFTR Research Margarida D. Amaral 18. Primary Epithelial Cell Models for Cystic Fibrosis Research Scott H. Randell, M. Leslie Fulcher, Wanda O'Neal, and John C. Olsen 19. Comparative Biology of Cystic Fibrosis Animal Models John T. Fisher, Yulong Zhang, and John F. Engelhardt 20. CFTR Folding Consortium: Methods Available for Studies of CFTR Folding and Correction Kathryn W. Peters, Tsukasa Okiyoneda, William E. Balch, Ineke Braakman, Jeffrey L. Brodsky, William B. Guggino, Christopher M. Penland, Harvey B. Pollard, Eric J. Sorscher, William R. Skach, Philip J. Thomas, Gergely L. Lukacs, and Raymond A. Frizzell 21. Evaluation of the Disease Liability of CFTR Variants Patrick R. Sosnay, Carlo Castellani, Mary Corey, Ruslan Dorfman, Julian Zielenski, Rachel Karchin, Christopher M. Penland, and Garry R. Cutting