Antibody Methods and Protocols (Hardcover, 2012)

1. Antigen Presentation for the Generation of Binding Molecules Hilmar Ebersbach, Gabriele Proetzel, and Chonghui Zhang 2. Recombinant Antibodies and In Vitro Selection Technologies C. Ronald Geyer, John McCafferty, Stefan Dubel, Andrew R.M. Bradbury, and Sachdev S. Sidhu 3. Phage Display Konstantin Petropoulos 4. Generation of Human Fab Libraries for Phage Display Christoph Rader 5. Selection of Human Fab Libraries by Phage Display Christoph Rader 6. Ribosome Display George Thom and Maria Groves 7. Hybridoma Technology for the Generation of Monoclonal Antibodies Chonghui Zhang 8. The Application of Transgenic Mice for Therapeutic Antibody Discovery E-Chiang Lee and Michael Owen 9. Production of Human or Humanized Antibodies in Mice Brice Laffleur, Virginie Pascal, Christophe Sirac, and Michel Cogne 10. Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies Elisabetta Traggiai 11. Kinetic Screening in the Antibody Development Process Michael Schraml and Matthias Biehl 12. Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection Michael Schraml and Leopold von Proff 13. Determination of Antibody Glycosylation by Mass Spectrometry Christiane Jager, Claudia Ferrara, Pablo Umana, Anne Zeck, Jorg Thomas Regula, and Hans Koll 14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format Jiahui Yang and Christoph Rader 15. PEGylation of Antibody Fragments for Half-Life Extension Simona Jevsevar, Mateja Kusterle, and Maja Kenig 16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann 17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann 18. Methods to Engineer and Identify IgG 1 Variants with Improved FcRn Binding or Effector Function Robert F. Kelley and Y. Gloria Meng 19. Class-Specific Effector Functions of Therapeutic Antibodies Virginie Pascal, Brice Laffleur, and Michel Cogne Konstantin Petropoulos 4. Generation of Human Fab Libraries for Phage Display Christoph Rader 5. Selection of Human Fab Libraries by Phage Display Christoph Rader 6. Ribosome Display George Thom and Maria Groves 7. Hybridoma Technology for the Generation of Monoclonal Antibodies Chonghui Zhang 8. The Application of Transgenic Mice for Therapeutic Antibody Discovery E-Chiang Lee and Michael Owen 9. Production of Human or Humanized Antibodies in Mice Brice Laffleur, Virginie Pascal, Christophe Sirac, and Michel Cogne 10. Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies Elisabetta Traggiai 11. Kinetic Screening in the Antibody Development Process Michael Schraml and Matthias Biehl 12. Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection Michael Schraml and Leopold von Proff 13. Determination of Antibody Glycosylation by Mass Spectrometry Christiane Jager, Claudia Ferrara, Pablo Umana, Anne Zeck, Jorg Thomas Regula, and Hans Koll 14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format Jiahui Yang and Christoph Rader 15. PEGylation of Antibody Fragments for Half-Life Extension Simona Jevsevar, Mateja Kusterle, and Maja Kenig 16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann 17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann 18. Methods to Engineer and Identify IgG 1 Variants with Improved FcRn Binding or Effector Function Robert F. Kelley and Y. Gloria Meng 19. Class-Specific Effector Functions of Therapeutic Antibodies Virginie Pasca