Crispr: Biology and Applications (Hardcover)

Contributors Vii

Preface xi

S E C T I O N I

Diversity of CRISPR‐Cas Systems 1

1 CRISPR-Cas Systems: Core Features and Common Mechanisms 3
Rodolphe Barrangou, Luciano A. Marraffini, and Erik J. Sontheimer

2 Evolutionary Classification of CRISPR-Cas Systems 13
Kira S. Makarova, Yuri I. Wolf, and Eugene V. Koonin

3 Molecular Mechanisms of Type I CRISPR-Cas Systems 39
John van der Oost

4 Molecular Mechanisms of Type II CRISPR‐Cas Systems 53
Tautvydas Karvelis and Virginijus Siksnys

5 Mechanism of Type III CRISPR‐Cas Immunity 71
Luciano A. Marraffini

6 Type V CRISPR‐Cas Systems 85
Morgan Quinn Beckett, Anita Ramachandran, and Scott Bailey

7 CRISPR-Cas13: Biology, Mechanism, and Applications of RNA-Guided, RNA-Targeting CRISPR Systems 99
Omar O. Abudayyeh and Jonathan S. Gootenberg

S E C T I O N II

CRISPR‐Cas Biology 121 8 CRISPR-Cas, Horizontal Gene Transfer, and the Flexible (Pan) Genome 123
Uri Gophna

9 Evasion Tactics Manifested by Bacteriophages against Bacterial Immunity 131
Jenny Y. Zhang, Sutharsan Govindarajan, and Joseph Bondy-Denomy

10 Regulation of CRISPR‐Cas Expression and Function 145
Leah M. Smith, Aroa Rey Campa, and Peter C. Fineran

S E C T I O N III

CRISPR‐Based Technologies and Applications 163

11 Genome Editing with CRISPR‐Cas Systems 165
Peter Lotfy and Patrick D. Hsu

12 Genetic and Epigenetic Modulation of Gene Expression by CRISPR-dCas Systems 195
Jasprina N. Noordermeer, Crystal Chen, and Lei S. Qi

13 CRISPR Screens 213
Jonathan D. D’Gama, Joseph S. Park, and Matthew K. Waldor

14 CRISPR‐Based Antimicrobials 233
Justen Russell and David Bikard

15 Exploiting CRISPR‐Cas Systems To Provide Phage Resistance in Industrial Bacteria 249
Rodolphe Barrangou and Avery Roberts

16 Recording Biological Information with CRISPR‐Cas Systems 267
Mariia Y. Cherepkova, Tanmay Tanna, and Randall J. Platt

Afterword 281

Index 283