Gene Knockout Protocols (Hardcover, 2, 2009)

Chapter 1 Overview on Mouse Mutagenesis Ralf Kuhn and Wolfgang Wurst Section I. Gene Modification in ES Cells Chapter 2 Construction of Gene Targeting Vectors by Recombineering Song-Choon Lee, Wei Wang, and Pentao Liu Chapter 3 Gene Trap Vectors and Mutagenesis Silke De-Zolt, Joachim Altschmied,, Patricia Ruiz, Harald von Melchner, and Frank Schnutgen Chapter 4 Chromosome Engineering in ES Cells Louise van der Weyden, Charles Shaw-Smith, and Allan Bradley Chapter 5 Gene Modification in Embryonic Stem Cells by Single-Stranded DNA Oligonucleotides Marieke Aarts, Marleen Dekker, Rob Dekker, Sandra de Vries, Anja van der Wal, Eva Wielders, and Hein te Riele Chapter 6 Generation of shRNA Transgenic Mice Christiane Hitz, Patricia Steuber-Buchberger,Sabit Delic, Wolfgang Wurst, and Ralf Kuhn Chapter 7 Mutagenesis of Mouse Embryonic Stem Cells with Ethylmethanesulfonate Robert Munroe and John Schimenti Section II. Stem Cell Manipulation Chapter 8 Gene Targeting in Mouse Embryonic Stem Cells Lino Tessarollo, Mary Ellen Palko, Keiko Akagi, and Vincenzo Coppola Chapter 9 Manipulating Mouse Embryonic Stem Cells Eileen Southon and Lino Tessarollo Chapter 10 ES Cell Line Establishment Heidrun Kern and Branko Zevnik Chapter 11 Generation of Double Knockout Embryonic Stem Cells Eva Wielders, Marleen Dekker, and Hein te Riele Chapter 12 Differentiation Analysis of Pluripotent Mouse Embryonic Stem (ES) Cells in vitro Insa S. Schroeder, Cornelia Wiese, Thuy T.Truong, Alexandra Rolletschek, and Anna M. Wobus Chapter 13 Cloning of ES cells and Mice by Nuclear Transfer Sayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama Section III. Genetically Engineered Mice Chapter 14 Isolation, Microinjection, and Transfer of Mouse Blastocysts Susan W. Reid and Lino Tessarollo Chapter 15 Aggregation Chimeras: Combining ES Cells, Diploid and Tetraploid Embryos Mika Tanaka, Anna-Katerina Hadjantonakis, Kristina Vintersten, and Andras Nagy Chapter 16 VelociMouse: Fully ES Cell-Derived F0 Generation Mice Obtained from the Injection of ES Cells into 8-Cell Stage Embryos Thomas M. DeChiara, William T. Poueymirou, Wojtek Auerbach, David Frendewey, George D. Yancopoulos, and David M. Valenzuela Chapter 17 Generation of Cre Recombinase Expressing Transgenic Mice Using Bacterial Artificial Chromosomes Jan Rodriguez Parkitna, David Engblom, and Gunther Schutz Chapter 18 Inducible Cre Mice Susanne Feil, Nadejda Valtcheva, and Robert Feil Chapter 19 Creation and Use of a Cre Recombinase Transgenic Database Andras Nagy, Lynn Mar, and Graham Watts Chapter 20 Transposon Mutagenesis in Mice David A. Largaespada Chapter 21 Lentiviral Transgenesis Alexander Pfeifer and Andreas Hofmann Chapter 22 Sperm Cryopreservation and In Vitro Fertilization Susan Marschall, Auke Boersma, and Martin Hrabe de Angelis Section IV. Phenotype Analysis Chapter 23 Influence of Genetic Background on Genetically Engineered Mouse Phenotypes Thomas