Antibody Engineering (Paperback)

50 chapters describing protocols in the following areas (Part titles): Introduction.- Generation of recombinant antibodies from hybridoma.- Generation of antibody libraries.- Isolation of antibody fragments from combinatorial libraries.- Expression and purification of antibody fragments in bacteria.- Expression and purification of antibody fragments in eukaryotic cells.- Determination of affinities.- Sequence and structure analysis and modelling epitope mapping.- In vivo experiments.- Affinity maturation of antibody fragments.- Humanisation of antibody fragments.- Antibody engineering to improve stability.- Bivalent and bispecific antibody fragments.- Recombinant antibody-fusion proteins.- Intracellular targeting of antibody fragments.The complete table of contents can be found on the Internet: http://www.springer.de