Laboratory Manual for Biotechnology and Laboratory Science : The Basics, Revised Edition (Paperback)

UNIT I SAFETY IN THE LABORATORYUnit Introduction SAFETY PART 1: CREATING A SAFE WORKPLACE Fundamental Principles Classroom Activity 1: Performing a Risk Assessment Classroom Activity 2: Exploring Safety-Related Government Web SitesClassroom Activity 3: Responding to Emergencies SAFETY PART 2: WORKING SAFELY WITH CHEMICALS Fundamental Principles Classroom Activity 4: Understanding the Chemicals with Which You WorkClassroom Activity 5: Personal Protection Laboratory Exercise 1:Tracking the Spread of Chemical Contamination Classroom Activity 6: Analyzing Safety Issues in a Laboratory ProcedureSAFETY PART 3: WORKING SAFELY WITH BIOLOGICAL HAZARDS Fundamental Principles Laboratory Exercise 2: Production of Bioaerosols and Factors Affecting Aerosol Production Unit DiscussionSafety Contract, student copySafety Contract, to turn in UNIT II DOCUMENTATION IN THE LABORATORY Unit Introduction Classroom Activity 7: Being an AuditorLaboratory Exercise 3:Keeping a Laboratory Notebook Classroom Activity 8: Writing and Following an SOP Unit DiscussionUNIT III METROLOGY IN THE LABORATORY Unit Introduction Laboratory Exercise 4: Recording Measurements with the Correct Number of Significant FiguresClassroom Activity 9: Constructing a Simple Balance Laboratory Exercise 5: Weight Measurements 1: Good Weighing Practices Laboratory Exercise 6:Weight Measurements 2: Performance Verification Laboratory Exercise 7: Volume Measurements 1: Proper Use of VolumeMeasuring Devices Laboratory Exercise 8: Volume Measurements 2: Performance Verification of a M Micropipette Laboratory Exercise 9: Measuring pH with Accuracy and Precision Unit Discussion UNIT IV SPECTROPHOTOMETRY AND THE MEASUREMENT OF LIGHT Unit Introduction Laboratory Exercise 10:Color and the Absorbance of LightLaboratory Exercise 11: Concentration, Absorbance, and TransmittanceLaboratory Exercise 12: Preparing a Standard Curve With Food Coloring and Using it forQuantitationClassroom Activity 10: Beer’s Law and Calculating an Absorptivity ConstantLaboratory Exercise 13: Determination of the Absorptivity Constant for ONPUnit Discussion UNIT V BIOLOGICAL SOLUTIONS Unit IntroductionClassroom Activity 11: Getting Ready to Prepare Solutions with One Solute: Calculations Classroom Activity 12: Getting Ready to Prepare Solutions with One Solute: Ordering ChemicalsLaboratory Exercise 14: Preparing Solutions with One SoluteLaboratory Exercise 15: Preparing Solutions to the Correct ConcentrationLaboratory Exercise 16: Working with BuffersLaboratory Exercise 17: Preparing Breaking Buffer Laboratory Exercise 18: Preparing TE Buffer Laboratory Exercise 19: More Practice Making a BufferLaboratory Exercise 20: Making a Quality Product in a Simulated CompanyUnit Discussion UNIT VI ASSAYSUnit Introduction Laboratory Exercise 21: Two Qualitative AssaysLaboratory Exercise 22: UV Spectrophotometric Assay of DNA: Quantitative Application Laboratory Exercise 23: UV Spectrophotometric Assay of DNA and Proteins: Qualitative ApplicationsLaboratory Exercise 24: The Bradford Protein Assay: Learning the AssayLaboratory Exercise 25: The Bradford Protein Assay: Exploring Assay VerificationLaboratory Exercise 26: The Beta-Galactosidase Enzyme AssayLaboratory Exercise 27:Comparing the Specific Activity of Two Preparations of Beta-GalactosidaseLaboratory Exercise 28: Using Spectrophotometry for Quality Control: NiacinUnit DiscussionUNIT VII BIOLOGICAL SEPARATION METHODS Unit Introduction Classroom Activity 13:Planning for Separating Materials Using a CentrifugeLaboratory Exercise 29: Separation of Two Substances Based on Their Differential Affinities for Two Phases Laboratory Exercise 30: Separation and Identification of Dyes Using Paper Chromatography Laboratory Exercise 31: Separating Molecules by Agarose Gel ElectrophoresisLaboratory Exercise 32: Using Agarose Gel Electrophoresis to Perform an AssayLaboratory Exercise 33: Optimizing Agarose Gel Electrophoresis Laboratory Exercise 34: Quantification of DNA by Agarose Gel ElectrophoresisLaboratory Exercise 35:Introduction to Ion Exchange Column Chromatography Unit Discussion UNIT VIII GROWING CELLS Unit Introduction GROWING CELLS PART I: BACTERIAL CELLS Laboratory Exercise 36: Using a Compound Light MicroscopeLaboratory Exercise 37: Aseptic Technique on an Open Lab Bench Laboratory Exercise 38: Working with Bacteria on an Agar Substrate: Isolating Individual Colonies Laboratory Exercise 39: Gram Staining Laboratory Exercise 40: Preparing Phosphate-Buffered SalineLaboratory Exercise 41: The Aerobic Spread-Plate Method of Enumerating Colony- Forming UnitsLaboratory Exercise 42: Preparing a Growth Curve for E. coli GROWING CELLS PART 2: MAMMALIAN CELLS Laboratory Exercise 43: Aseptic Technique in a Biological Safety CabinetLaboratory Exercise 44: Making Ham’s F12 Medium from Dehydrated PowderLaboratory Exercise 45: Examining, Photographing, and Feeding CHO CellsLaboratory Exercise 46: Counting Cells Using a HemocytometerLaboratory Exercise 47: Subculturing CHO Cells Laboratory Exercise 48: Preparing a Growth Curve for CHO Cells Unit Discussion APPENDICES 1.Abbreviations and Acronyms Used in this Laboratory Manual 2.Glossary 3.Selected Bibliography 4.Brief Metric Review 5.Calculating Standard Deviation 6.Equipment, Supplies, and Reagents Required for Each Unit 7. Recipes and Preparation Notes ?