Nucleic Acid Aptamers: Selection, Characterization, and Application (Hardcover)

· 제목 : Nucleic Acid Aptamers: Selection, Characterization, and Application (Hardcover) 
· 분류 : 외국도서 > 과학/수학/생태 > 과학 > 화학 > 화학 일반
· ISBN : 9781071626948
· 쪽수 : 282쪽

Part I: Selection

 

1. Automated ssDNA SELEX Using Histidine Tagged Target Proteins

            Stefan Breuers and Gunter Mayer

 

2. Discovery of Aptamers against Cell Surface Markers Using Ligand-Guided Selection

            Nicole Williams, Rutika Patel, and Prabodhika Mallikaratchy

 

3. Implementation of Emulsion PCR for Amplification of Click-Modified DNA during SELEX

            Moujab Choukeife, Anna Jonczyk, and Gunter Mayer

 

4. Preparation of Chemically Modified DNA Library for SELEX via Incorporation of CLB-dUTP in Primer Extension Reaction

            Magda Puchała, Marta Radzi?ska, Joanna Guzdek, Agnieszka Sok-Grochowska, Aleksandra Adamowicz-Skrzypkowska, Paweł Pi?ta, Przemysław Jurek, and Monika Czarnecka

 

5. RNA Capture-SELEX on Streptavidin Magnetic Beads

            Leon Kraus and Beatrix Suess

 

6. The Bioinformatics of Aptamers: HT-SELEX Analysis with AptaSUITE

            Jan Hoinka and Teresa M. Przytycka

 

7. Improvement of Aptamers by High-Throughput Sequencing of Doped-SELEX

            Frederic Duconge

 

Part II: Characterization

 

8. Analysis of Aptamer-Small Molecule Binding Interactions Using Isothermal Titration Calorimetry

            Sladjana Slavkovic and Philip E. Johnson

 

9. Determination of Aptamer Structure Using Circular Dichroism Spectroscopy

            Yannick Kerler, Stephan Sass, Carsten Hille, and Marcus M. Menger

 

10. Immobilization-Free Determination of Dissociation Constants Independent of Ligand Size Using MicroScale Thermophoresis

            Wiebke Sabrowski, Walter F.M. Stocklein, and Marcus M. Menger

 

11. Label-Free Determination of the Kinetic Parameters of Protein-Aptamer Interaction by Surface Plasmon Resonance

            Nico Dreymann, Anja Moller, and Marcus M. Menger

 

12. Probing of Fluorogenic RNA Aptamers via Supramolecular Forster Resonance Energy Transfer with Universal Fluorescent Nucleobase Analog

            Christian Steinmetzger and Claudia Hobartner

 

13. Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT

            Sebastian Strauss and Ralf Jungmann

 

Part III: Application

 

14. Aptamer-Field-Effect Transistors for Small-Molecule Sensing in Complex Environments

            Nako Nakatsuka

 

15. Aptasensor for Impedimetric Detection of Lysozyme

            Ece Eksin, Huseyin Senturk, and Arzum Erdem

 

16. Biophysical Analysis of Small Molecule Binding to Viral RNA Structures

            Liang-Yuan Chiu, Jesse Davila-Calderon, Zhengguo Cai, and Blanton S. Tolbert

 

17. Designing and Expressing Circular RNA Aptamers to Regulate Mammalian Cell Biology

            Jacob L. Litke and Samie R. Jaffrey

 

18. Enzyme-Linked OligoNucleotide Assay (ELONA)

            Miguel Moreno, Ana Garcia-Sacristan, M. Elena Martin, and Victor M. Gonzalez

 

19. High-Throughput Development and Optimization of RNA-Based Fluorogenic Biosensors of Small Molecules Using Droplet-Based Microfluidics

            Claire Husser, Stephanie Baudrey, and Michael Ryckelynck

 

20. Robotic APTamer-Enabled Electrochemical Reader (RAPTER) System for Automated Aptamer-Mediated Electrochemical Analysis

            Young Lo, Shaolin Liang, Waljit S. Dhillo, Anthony E.G. Cass, and Julian A. Tanner