Principles and Reactions of Protein Extraction, Purification, and Characterization (Paperback, 2)

Extraction of Protein
Preparation of Buffers for Protein Extraction
Use of Protease Inhibitors in Extraction
Use of Detergents in Extraction
Chemical Lysis for Protein Extraction
Mechanical Lysis for Protein Extraction
Preparation of Extracts from Prokaryotes
Preparation of Extracts from Yeast
Preparation of Extracts from Eukaryotes
Preparation of Extracts from Plants
Preparation of Membrane Extracts
Preparation and Extraction of Recombinant Protein
Take-Home Message
References
Estimation of Protein
Ultraviolet Absorption Methods
Colorimetric Methods
Fluorescent Methods
Take-Home Message
References
Electrophoretic Analyses of Protein
Driving Force of Electrophoresis
Polyacrylamide Gel Electrophoresis
3.3 Isoelectric Focusing
Two-Dimensional (2D) Gel Electrophoresis
Western Blotting
Capillary Electrophoresis
Take-Home Message
References
Purification of Protein
General Consideration and Purification Strategy
Non-Chromatographic Purification of Proteins
Chromatographic Purification of Proteins
Take-Home Message
References
Antibodies: Structures, Interactions, and Production
Immune System and Antibody Response
Structure of Antibodies
Antigen-Antibody Interactions
Production of Antibodies
Development of Monoclonal Antibodies
Purification of Antibodies
Take-Home Message
References
Antibody Labeling, Antibody Detection, and Immunochemical Techniques
Methods of Labeling Antibodies
Detection of Antibody Binding
Inununochemical Techniques: A Wide Range ofInununoassays for
Detecting and Quantitating (Semi) Antigen-Antibody Reactions
Immunoprecipitation
Immunohistochemistry
Flow Cytometry
Take-Home Message
References
Identification and Annotation of the Target Protein
Enzymatic Cleavage of Protein
Chemical Cleavage
Sequencing Peptides
Identification of Protein by Mass Spectrometry
Annotation by Protein Database Search
Take-Home Message
References
Identification of Most Common Post-translational Modification (Glycosylation): Purification of Glycoproteins and Analyses of Their Oligosaccharides
Some Basics of Carbohydrates
Is My Protein Glycosylated?
Purification of Glycoproteins
Analyses of Carbohydrate Structures
Take-Home Message
References
Identification of Other Posttranslational Modifications
Phosphorylation
Palmitoylation
Myristoylation
GPI Modification
Acetylation
Methylation
Transamidation
Ubiquitination
Hydroxylation of Proline and Lysine
Proteolytic Degradation
Take-Home Message
References
Index