Protein Affinity Tags: Methods and Protocols (Paperback, Softcover Repri)

1. 1D4 - A Versatile Epitope Tag for the Purification and Characterization of Expressed Membrane and Soluble Proteins Laurie L. Molday and Robert S. Molday 2. Affinity Purification of Heme-Tagged Proteins Wesley B. Asher and Kara L. Bren 3. Purification of a Recombinant Protein with Cellulose-Binding Module 3 as the Affinity Tag Dongmei Wang and Jiong Hong 4. Purification of E. coli Proteins Using a Self-Cleaving Chitin-Binding Affinity Tag Michael J. Coolbaugh and David W. Wood 5. Simplified Protein Purification Using an Autoprocessing, Inducible Enzyme Tag Aimee Shen 6. SUMO as a Solubility Tag and in vivo Cleavage of SUMO Fusion Proteins with Ulp1 Dennis Kuo, Minghua Nie, and Albert J. Courey 7. Rescuing Aggregation-Prone Proteins in Escherichia coli with a Dual His6-MBP Tag Danielle Needle and David S. Waugh 8. Expression, Purification, and Immobilization of Recombinant Tamavidin 2 Fusion Proteins Yoshimitsu Takakura, Naomi Oka, and Masako Tsunashima 9. Use of Tandem Affinity Chromatography for Purification of Cannabinoid Receptor CB2 Silvia C. Locatelli-Hoops and Alexei A. Yeliseev 10. Detection of Protein-Protein Interactions Using Tandem Affinity Purification Ian Goodfellow and Dalan Bailey 11. An Improved in vivo Biotinylation Strategy Combined with FLAG and Antibody Based Approaches for Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells Francesco Faiola, Arven Saunders, Baoyen Dang, and Jianlong Wang 12. Purification of Recombinant Proteins with a Multifunctional GFP Tag Takashi Murayama and Takuya Kobayashi 13. Targeted Purification of SnAvi-Tagged Proteins Ursula Schaffer, Ralf Baumeister, and Ekkehard Schulze 14. An Efficient Fluorescent Protein-Based Multifunctional Affinity Purification Approach in Mammalian Cells Hanhui Ma, Janel R. McLean, Kathleen L. Gould, and Dannel McCollum 15. Bimolecular Affinity Purification - A Variation of TAP with Multiple Applications Petro Starokadomskyy and Ezra Burstein