Cell-Free Protein Synthesis: Methods and Protocols (Paperback, Softcover Repri)

1. Production of Eukaryotic Cell-Free Lysate from Leishmania tarentolae Wayne Johnston and Kirill Alexandrov 2. Bioinformatics Analysis and Optimization of Cell-Free Protein Expression Alexander A. Tokmakova, Atsushi Kurotania, Mikako Shirouzu, Yasuo Fukamid, and Shigeyuki Yokoyama 3. A Cell-Free Expression Screen to Identify Fusion Tags for Improved Protein Expression Andrew Kralicek 4. One-Pot, Microscale Cell-Free Enzyme Expression and Screening Aarthi Chandrasekaran and Anup K. Singh 5. Cell-Free Translation of Biofuels Enzymes Taichi E. Takasuka, Johnnie A. Walker, Lai F. Bergeman, Kirk A. Vander Meulen, Shin-ichi Makino, Nathaniel L. Elsen, and Brian G. Fox 6. Cloning-Independent Expression and Screening of Enzymes using Cell-Free Protein Synthesis Systems Yong-Chan Kwon, Jae-Kwang Song, and Dong-Myung Kim 7. High Level Cell-Free Production of Membrane Proteins with Nanodiscs Christian Roos, Lei Kai, Stefan Haberstock, Davide Proverbio, Umesh Ghoshdastider, Yi Ma, Slawomir Filipek, Xiaoning Wang, Volker Dotsch, and Frank Bernhard 8. Cell Free Protein Based Enzyme Discovery and Protein-Ligand Interaction Study Sabrina Guillemer, Cecile Persillon, Jean-Michel Masson, and Gilles Ravot 9. Human Cell Extract-Derived Cell-Free Systems for Virus Synthesis Tominari Kobayashi, Kodai Machida, and Hiroaki Imataka 10. Cell-Free Protein Synthesis in Microfluidic 96-Well Plates Kirsten Jackson, Ruba Khnouf, and Z. Hugh Fan 11. Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein Synthesis Kiyoshi Ozawa and Ruhu Qi 12. Site-Specific Incorporation of Unnatural Amino Acids into Proteins by Cell-Free Protein Synthesis Kiyoshi Ozawa and Choy Theng Loh 13. In vitro Translation of Papillomavirus Authentic and Codon-Modified L1 Capsid Gene mRNAs in Mouse Keratinocyte Cell-Free Lysate Kong-Nan Zhao 14. An Optimized Yeast Cell-Free Lysate System for In vitro Translation of Human Virus mRNA Xiao Wang, Liang Zhao, and Kong-Nan Zhao 15. In vitro Translation Based Protein Kinase Substrate Identification Szilvia K. Nagy and Tamas Meszaros 16. Preparation of Protein Arrays using Cell-Free Protein Expression Elizabeth A. Cook and Mingyue He 17. Post-Transcriptional Control of Protein Synthesis in Drosophila S2 Cell-Free System Motoaki Wakiyama and Shigeyuki Yokoyama 18. Cell-Free Membrane Protein Expression Tomomi Kimura-Soyema, Mikako Shirouzu, and Shigeyuki Yokoyama 19. The PURE System for Protein Production Yoshihiro Shimizu, Yutetsu Kuruma, Takashi Kanamori, and Takuya Ueda 20. A Cell-Free Protein Synthesis System from Insect Cells Toru Ezure, Takashi Suzuki, and Eiji Ando 21. A Cell-Free Expression Platform for Production of Protein Microarrays Xristo Zarate and David W. Galbraith