Protein-Carbohydrate Interactions: Methods and Protocols (Paperback, Softcover Repri)

Preface... Table of Contents... Contributing Authors... Part I Analysis of Carbohydrate-Active Enzyme Activity 1. A Low-Volume, Parallel Copper-Bicinchoninic Acid (BCA) Assay for Glycoside Hydrolases Gregory Arnal, Mohamed A. Attia, Jathavan Asohan, and Harry Brumer 2. Quantitative Kinetic Characterization of Glycoside Hydrolases using High-Performance Anion-Exchange Chromatography (HPAEC) Nicholas McGregor, Gregory Arnal, and Harry Brumer 3. Measuring Enzyme Kinetics of Glycoside Hydrolases using the 3,5-Dinitrosalicylic Acid Assay Lauren S. McKee 4. An Improved Kinetic Assay for the Characterization of Metal-Dependant Pectate Lyases Darryl R. Jones, Richard McLean, and D. Wade Abbott 5. Colorimetric Detection of Acetyl Xylan Esterase Activities Galina Mai-Gisondi and Emma Master 6. Methods for Determining Glycosyltransferase Kinetics Maria Ngo and Michael D.L. Suits 7. Analyzing Activities of Lytic Polysaccharide Monooxygenases by Liquid Chromatography and Mass Spectrometry Bjørge Westereng, Magnus Ø. Arntzen, Jane Wittrup Agger, Gustav Vaaje-Kolstad, and Vincent G.H. Eijsink 8. Carbohydrate Depolymerization by Intricate Cellulosomal Systems Johanna Stern, Lior Artzi, Sarah Morais, Carlos M.G. A. Fontes, and Edward A. Bayer Part II Analysis of Protein-Carbohydrate Interactions 9. Affinity Electrophoresis for Analysis of Catalytic Module-Carbohydrate Interactions Darrell Cockburn, Casper Wilkens, and Birte Svensson 10. Quantifying CBM Carbohydrate Interactions using Microscale Thermophoresis Haiyang Wu, Cedric Y. Montanier, and Claire Dumon 11. Characterization of Protein-Carbohydrate Interactions by NMR Spectroscopy Julie M. Grondin, David N. Langelaan, and Steven P. Smith < 12. Measuring the Biomechanical Loosening Action of Bacterial Expansins on Paper and Plant Cell Walls Daniel J. Cosgrove, Nathan K. Hepler, Edward R. Wagner, and Daniel M. Durachko Part III Visualization of Carbohydrates and Protein-Carbohydrate Complexes 13. Bioinspired Assemblies of Plant Cell Walls for Measuring Protein-Carbohydrate Interactions by FRAP Gabriel Paes 14. CBMs as Probes to Explore Plant Cell Wall Heterogeneity using Immunocytochemistry Louise Badruna, Vincent Burlat, and Cedric Y. Montanier 15. Determining the Localization of Carbohydrate Active Enzymes within Subcellular Compartments of Gram-Negative Bacteria Richard McLean, G. Douglas Inglis, Steven C. Mosimann, Richard R. Uwiera, and D. Wade Abbott 16. Analysis of Complex Carbohydrate Composition in Plant Cell Wall using Fourier Transformed Mid-Infrared Spectroscopy (FT-IR) Ajay Badhan, Yuxi Wang, and Tim A. McAllister 17. Separation and Visualization of Glycans by Fluorophore-Assisted Carbohydrate Electrophoresis Melissa Robb, Joanna K. Hobbs, and Alisdair B. Boraston 18. A Rapid Procedure for the Purification of 8-Aminopyrene Trisulfonate (APTS)-Labelled Glycans for Capillary Electrophoresis (CE)-Based Enzyme Assays Hayden J. Danyluk, Leona K. Shum, and Wesley F. Zandberg Part IV CAZyme Structure, Discovery, and Prediction Methods 19. Probing the Complex Architecture of Multimodular Carbohydrate-Active Enzymes using a Combination of Small Angle X-Ray Scattering and X-Ray Crystallography Mirjam Czjzek and Elizabeth Ficko-Blean 20. Metagenomics and CAZyme Discovery B.J. Kunath, A. Bremges, A. Weimann, A.C. McHardy, and P.B. Pope 21. Identification of Genes Involved in the Degradation of Lignocellulose using Comparative Transcriptomics R.J. Gruninger, I. Reid, R.J. Forster, A. Tsang, and T.A. McAllister 22. Isolation and Preparation of Extracellular Proteins from Lignocellulose Degrading Fungi for Comparative Proteomic Studies using Mass Spectrometry R.J. Gruninger, A. Tsang, and T.A. McAllister