Membrane Protein Structure and Function Characterization: Methods and Protocols (Paperback, Softcover Repri)

1. Recombinant Overexpression of Mammalian TSPO Isoforms 1 and 2 Lucile Senicourt, Soria Iatmanen-Harbi, Claude Hattab, Mariano A Ostuni, Marie-France Giraud, and Jean-Jacques Lacapere 2. Functional Assembly of Soluble and Membrane Recombinant Proteins of Mammalian NADPH Oxidase Complex Hajer Souabni, Aymen Ezzine, Tania Bizouarn, and Laura Baciou 3. Direct Extraction and Purification of Recombinant Membrane Proteins from Pichia pastoris protoplasts Lucie Hartmann, Estelle Metzger, Noemie Ottelard, and Renaud Wagner 4. Cell-free Expression for the Study of Hydrophobic Proteins: The Example of Yeast ATP-synthase subunits Isabelle Larieu, James Tolchard, Corinne Sanchez, Edmond Yazo Kone, Alexandre Barras, Claire Stines-Chaumeil, Benoit Odaert, and Marie-France Giraud 5. Wheat Germ Cell Free Overexpression for the Production of Membrane Proteins Marie-Laure Faugeron, Aurelie Badillo, Francois Penin, and Anja Bocjmann 6. Methyl-specific Isotope Labelling Strategies for NMR Studies of Membrane Proteins Vilius Kurauskas, Paul Schanda, and Remy Sounier 7. Labeling of Membrane Complexes for Electron Microscopy Francesca Gubellini and Remi Fronzes 8. Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins Case Study of The Mouse 5-HT Receptor Gherici Hassaine, Cedric Deluz, Luigino Grasso, Romain Wyss, Ruud Hovius, Henning Stahlberg, Takashi Tomizaki, Aline Desmyter, Christophe Moreau, Lucie Peclinovska, Sonja Minniberger, Lamia Mebarki, Xiao-Dan Li, Horst Vogel, and Hugues Nury 9. Characterization of New Detergent and Detergent Mimetics by Scattering Techniques for Membrane Protein Crystallization Francoise Bonnete and Patrick J Loll 10. Secondary Structure Determination by Means of ATR-FTIR Spectroscopy Batoul Srour, Stefan Bruechert, Susana Andrade, and Petra Hellwig 11. Native Mass Spectroscopy for the Characterization of Structure and Interactions of Membrane Proteins Jeroen F Van Dyck, Albert Konijnenberg, and Frank Sobott 12. Mass Spectroscopy of Mitochondrial Membrane Proteins Complexes Luc NEGRONI, Michel ZIVY, and Claire LEMAIRE 13. Functional Studies on Membrane Proteins by Means of H/D Exchange in Infrared: Structural Changes in Na + NQR from V cholerae in the Presence of Lipids Yashvin Neehaul, Sebastien Kriegel, Blanca Barquera, and Petra Hellwig 14. Reconstitution of Membrane Protein in Liposomes Alice Verchere, Isabelle Broutin, and Martin Picard 15. Ion Channels as Reporters of Membrane Receptor Function: Automated Analysis in Xenopus Oocytes Michel Vivaudou, Zlatomir Todorov, Gina Catalina Reyes-Mejia, and Christophe Moreau 16. The CRACAM Robot: Two Dimensional Crystallization of Membrane Protein Philippe Rosier, Frederic Gelebart, Nicolas Dumesnil, Gauthier Esnot, Manuela Dezi, Marc Morand, and Catherine Venien-Bryan 17. Reconstitution of Membrane Proteins into Nanodiscs for Single Particle Electron Microscopy Laetitia Daury, Jean-Christophe Taveau, Dimitri Salvador, Marie Glavier, and Olivier Lambert 18. Solid-state NMR of Membrane Protein Reconstituted in Proteoliposomes, the Case of TSPO Lucile Senicourt, Luminita Duma, Vassilios Papadopoulos, and Jean-Jacques Lacapere 19. Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR Denis Lacabanne, Britta Kunert, Carole Gardiennet, Beat H Meier, and Anja Bockmann 20. Simulation of Ligand Binding To Membrane Proteins Samuel Murail 21. Molecular modelling of Transporters: From Low Resolution Cryo-Electron Microscopy Map to Conformational Exploration. The Example of TSPO Molecular Dynamic of Membrane Protein in Different Environments Aurore Vaitinadapoule and Catherine Etchebest