Antimicrobial Peptides: Methods and Protocols (Hardcover)

Part I: Isolation, Purification, Design, and Synthesis of Antimicrobial Peptides 1. Antimicrobial Peptides in Frog Skin Secretions J. Michael Conlon and Agnes Sonnevend 2. Purification of Antimicrobial Peptides from Human Skin Jens-M. Schroder 3. Strategies for the Isolation and Characterization of Antibacterial Lantibiotics Daniela Jabes and Stefano Donadio 4. Expression and Purification of Recombinant a -Defensins and a -Defensin Precursors in Escherichia coli Sharel Figueredo, Jennifer R. Mastroianni, Kenneth P. Tai, and Andre J. Ouellette 5. Production of Recombinant Antimicrobial Peptides in Bacteria Mateja Zorko and Roman Jerala 6. Methods for Building Quantitative Structure-Activity Relationship (QSAR) Descriptors and Predictive Models for Computer-Aided Design of Antimicrobial Peptides Olivier Taboureau 7. Synthesis and Thermodynamic Characterization of Small Cyclic Antimicrobial Arginine and Tryptophan-Rich Peptides with Selectivity for Gram-Negative Bacteria Mojtaba Bagheri 8. Synthesis of Antimicrobial Peptides Using the SPOT Technique Dirk F.H. Winkler and Kai Hilpert 9. High-Throughput Screening for Antimicrobial Peptides Using the SPOT Technique Kai Hilpert Part II: Analysis, Properties, and Mechanisms of Antimicrobial Peptides 10. Antimicrobial Peptides: The LPS Connection Andrea Giuliani, Giovanna Pirri, and Andrea C. Rinaldi 11. Binding and Permeabilization of Model Membranes by Amphipathic Peptides Paulo F. Almeida and Antje Pokorny 12. Comparing Bacterial Membrane Interactions of Antimicrobial Peptides and Their Mimics Nathaniel P. Chongsiriwatana and Annelise E. Barron 13. Dynamic Transitions ofMembrane-Active Peptides Stephan L. Grage, Sergii Afonin, and Anne S. Ulrich 14. Solid-State NMR Investigations of Membrane-Associated Antimicrobial Peptides Christopher Aisenbrey, Philippe Bertani, and Burkhard Bechinger 15. Use of Atomic Force Microscopy as a Tool to Understand the Action of Antimicrobial Peptides on Bacteria Ang Li, Bow Ho, Jeak Ling Ding, and Chwee Teck Lim 16. Fluorescence and Electron Microscopy Methods for Exploring Antimicrobial Peptides Mode(s) of Action Ludovica Marcellini, Maria Giammatteo, Pierpaolo Aimola, and Maria Luisa Mangoni 17. Molecular Simulations of Antimicrobial Peptides Allison Langham and Yiannis N. Kaznessis 18. Computer-Based Analysis, Visualization, and Interpretation of Antimicrobial Peptide Activities Ralf Mikut Part III: Antimicrobial Peptides as Therapeutic Agents 19. Potential Therapeutic Application of Host Defense Peptides Lijuan Zhang and Timothy J. Falla 20. Therapeutic Potential of HDPs as Immunomodulatory Agents Havard Jenssen and Robert E.W. Hancock 21. Assay Systems for Measurement of Anti-Inflammatory Activity Evelina Rubinchik and Christopher Pasetka 22. Ex-Vivo Skin Infection Model Evelina Rubinchik and Christopher Pasetka 23. Measuring Antimicrobial Peptide Activity on Epithelial Surfaces in Cell Culture Gill Diamond, Sunghan Yim, Isaura Rigo, and Laura McMahon 24. Antimicrobial and Antibiofilm Activity of Quorum Sensing Peptides and Peptide Analogues Against Oral Biofilm Bacteria Karen LoVetri and Srinivasa Madhyastha 25. Characterization of the Leishmanicidal Activity of Antimicrobial Peptides Juan Roman Luque-Ortega and Luis Rivas