Bioprocess Engineering (Hardcover)

Dedication

List of figures

List of plates

Preface

About the author

Chapter 1: Historical development: from ethanol to biopharmeceuticals

Abstract:

Chapter 2: Microbiology

Abstract:

2.1 Microorganisms: the core of cellular bioprocesses

2.2 Cellular structure and sites of metabolic reactions

2.3 Classification according to carbon and energy requirements

2.4 Nutrient requirements

Chapter 3: Metabolic macromolecules

Abstract:

3.1 Carbohydrates

3.2 Lipids

3.3 Proteins

3.4 Nucleosides, nucleotides and nucleic acids

Chapter 4: Molecular biology

Abstract:

4.1 Replication, transcription and translation

4.2 Genetic regulation

4.3 Genetic modification

Chapter 5: Carbon metabolism

Abstract:

5.1 Energy generation, storage and transfer

5.2 Catabolic pathways: energy generation

5.3 Anabolic pathways: energy utilisation

Chapter 6: Enzymes as biocatalysts

Abstract:

6.1 Enzyme kinetics with no inhibition

6.2 Enzyme kinetics with inhibition

6.3 Enzyme reactors with soluble enzymes

6.4 Enzyme reactors with immobilised enzymes

Chapter 7: Microbial kinetics during batch, continuous and fed-batch processes

Abstract:

7.1 The nutrient medium

7.2 Batch process design equations

7.3 Continuous process design equations

7.4 Fed-batch bioprocess design equations

Chapter 8: The oxygen transfer rate and overall volumetric oxygen transfer coefficient

Abstract:

8.1 Oxygen transfer design equations

8.2 Measurement of the oxygen transfer rate

Chapter 9: Bioprocess scale up

Abstract:

9.1 Scale up with constant oxygen transfer rate

9.2 Scale up with constant mixing

9.3 Scale up with constant shear stress

9.4 Scale up with constant flow regime

Chapter 10: Bioprocess asepsis and sterility

Abstract:

10.1 Heat sterilisation of media and equipment

10.2 Filter sterilisation of air

Chapter 11: Downstream processing

Abstract:

11.1 Overview of potential recovery operations

11.2 Separation of cells and extracellular fluid

11.3 Cell rupture and separation of cell extract

11.4 Concentration and purification of soluble products

Index