The Maize Handbook (Paperback, Softcover Repri)

1. Fertilization Embryogeny in Maize.- 2. The Maize Shoot.- 3. The Maize Leaf.- 4. The Maize Root.- 5. Morphology and Development of the Tassel and Ear.- 6. Gametogenesis in Maize.- 7. The Patterns of Plant Structures in Maize.- 8. Genetics and the Morphological Evolution of Maize.- 9. Overview of Key Steps in Aleurone Development.- 10. Light Microscopy I: Dissection Microtechnique.- 11. Light Microscopy II: Observation, Photomicrography, and Image Analysis.- 12. Scanning Electron Microscopy.- 13. Transmission Electron Microscopy: Chemical Fixation, Freezing Methods, and Immunolocalization.- 14. Techniques for Histology of Maize Megaspores and Embryo Sacs.- 15. Indirect Immunofluorescence: Localization of the Cytoskeleton.- 16. Immunocytochemistry for Light and Electron Microscopy.- 17. Immunolocalization of Nuclear Proteins.- 18. In situ Hybridization.- 19. Photography of Maize.- 20. Genetic Experiments Mapping.- 21. Growing Maize for Genetic Studies.- 22. A Nine-Step Way to Characterize a Morphological Mutant.- 23. Mutagenesis.- 24. Gene Tagging with Ac/Ds Elements in Maize.- 25. Using Cytogenetics to Enhance Transposon Tagging with Ac Throughout the Maize Genome.- 26. Transposon Tagging with Spm.- 27. Transposon Tagging with Mutator.- 28. Mapping Genes with Recombinant Inbreds.- 29. The Placement of Genes Using waxy-Marked Reciprocal Translocations.- 30. Chimeras for Genetic Analysis.- 31. The Use of Clonal Sectors for Lineage and Mutant Analysis.- 32. Use of Segmental Aneuploids for Mutant Analysis.- 33. Biased Transmission of Genes and Chromosomes.- 34. Anthocyanin Genetics.- 35. Cloned Anthocyanin Genes and Their Regulation.- 36. Maize and Puccinia sorghi-Marked: A System for the Study of the Genetic and Molecular Basis of Host-Pathogen Interactions.- 37. Disease Lesion Mutants.- 38. Classification of Pollen Abortion in the Field.- 39. Genetic Fine Structure as Revealed in Pollen Assays.- 40. Genetic Fine Structure from Testcross Progeny Analysis.- 41. Trisomic Manipulation.- 42. B-A Translocation Manipulation.- 43. Locating Recessive Genes to Chromosome Arm with B-A Translocations.- 44. Dosage Analysis Using B-A Translocations.- 45. Marker Systems for B-A Translocations.- 46. Construction of Compound B-A Translocations.- 47. A-B-A Compound Chromosomes.- 48. Comprehensive List of B-A Translocations in Maize.- 49. Chromosomal Translocations Involving the Nucleolus Organizer Region or Satellite of Chromosome 6.- 50. Inversions and List of Inversions Available.- 51. Use of Maize Monosomics for Gene Localization and Dosage Studies.- 52. Marker Systems for r-x1.- 53. Translocations as Genetic Markers.- 54. A-A Translocations: Breakpoints and Stocks.- 55. Segmental Aneuploid Analysis.- 56. Directed Synthesis of Segmental Transpositions.- 57. Practical Aspects of Haploid Production.- 58. Indeterminate Gametophyte (ig): Biology and Use.- 59. Production of a Ploidy Series.- 60. Absorption Cytophotometry of Nuclear DNA.- 61. Flow Cytometry for Endosperm Nuclear DNA.- 62. Allelism Testing of Lethal Mutations.- 63. Analysis of Cytoplasmically Inherited Mutants.- 64. Male Sterility and Restorer Genes in Maize.- 65. Inbred Lines of Maize and Their Molecular Markers.- 66. Traditional Analysis of Maize Pachytene Chromosomes.- 67. Techniques for Preparing Whole-Mount Spreads of Maize Pachytene Chromosome Complements for Electron-Microscopic Visualization of Synaptonemal Complex Structures.- 68. A Smear Technique for the Study of Meiosis in Pollen Mother Cells of Maize.- 69. Protocol for Preparing Maize Macrospore Mother Cells for the Study of Female Meiosis and Embryo-sac Development.- 70. Preparing a Suspension of Microsporocytes for Spreading and Electron Microscopy.- 71. Three-dimensional Fluorescence Microscopy of Maize Chromosomes.- 72. Chromosomal Behavior During Microsporogenesis.- 73. A Staining Procedure for Pollen Grain Chromosomes of Maize.- 74. A Technique for the Preparation of Somatic Chromosomes of Maize.- 75. A Technique for Somatic Chromosome Preparation and C-banding of Maize.- 76. Duplications.- 77. Deficiency Analysis.- 78. The Gametophyte Factors of Maize.- 79. Ring Chromosomes.- 80. In situ Hybridization of DNA and RNA Probes to Maize Chromosomes.- 81. Analysis of Traits with Complex Inheritance in Maize Using Molecular Markers.- 82. Heterofertilization.- 83. Isolation of Small Nuclear RNAs.- 84. Plant DNA Miniprep and Microprep: Versions 2.1?2.3.- 85. Urea-based Plant DNA Miniprep.- 86. High-Molecular-Weight Plant DNA Preparation for CHEF Gel Analysis.- 87. Preparation of High-Molecular-Weight Maize DNA and Analysis by Pulsed-Field Gel Electrophoresis.- 88. Isolation of Genomic DNA from Calli.- 89. Isolation of DNA from Immature Cobs.- 90. Preparation of Nucleic Acids from Maize Microspores and Pollen.- 91. Preparation of DNA and RNA from Leaves: Expanded Blades and Separated Bundle Sheath and Mesophyll Cells.- 92. Isolation of RNA from Wx and wx Endosperms.- 93. RNA Isolation from Electroporated Protoplasts.- 94. Procedures for Isolating Mitochondria and Mitochondrial DNA and RNA.- 95. Isolation of Maize Chloroplasts and Chloroplast DNA.- 96. In vitro Capping of Maize Mitochondrial RNA and Transcription Initiation Site Characterization by RNase Protection.- 97. Editors’ Note.- 98. Southern Blots of Maize Genomic DNA.- 99. Southern Blot Hybridization.- 100. Northern Blotting.- 101. RNase Protection Assay.- 102. Genomic Sequencing in Maize.- 103. The Polymerase Chain Reaction: Applications to Maize Transposable Elements.- 104. In vitro Synthesis of Capped and Polyadenylated mRNA for Translation Studies in vitro and in vivo.- 105. Construction of a Genomic Library in Lambda Phage.- 106. Agroinfection.- 107. Polyethylene Glycol-mediated DNA Uptake into Maize Protoplasts.- 108. DNA Delivery into Maize Cell Cultures Using Silicon Carbide Fibers.- 109. Transient Gene Expression Assay by Electroporation of Maize Protoplasts.- 110. Assay of Bacterial Chloramphenicol Acetyl Transferase in Transformed Maize Tissues.- 111. Assay of Firefly Luciferase in Transformed Maize Tissues.- 112. Storage of Frozen Maize Tissue.- 113. Maize Methods?Starch Biosynthetic Genes.- 114. Identifying and Characterizing the TATA Box Promoter Sequence Element in a Maize Nuclear Gene.- 115. Promoters.- 116. Introns.- 117. Characterization of Zein Genes and Their Regulation in Maize Endosperms.- 118. Overview of Cloning Genes Using Transposon Tagging.- 119. How RFLP Loci Can Be Used to Assist Transposon-Tagging Efforts.- 120. Regeneration of Plants from Somatic Cell Cultures: Applications for in vitro Genetic Manipulation.- 121. Initiation, Maintenance, and Plant Regeneration of Type II Callus and Suspension Cells.- 122. Production of Transgenic Maize Plants via Microprojectile-Mediated Gene Transfer.- 123. In vitro Selection.- 124. Selection of Stable Transformants from Black Mexican Sweet Maize Suspension Cultures.- 125. Maize Protoplast Culture.- 126. Anther and Microspore Culture.- 127. In vitro Culture of Maize Kernels.- 128. In vitro Ear Culture System.- 129. Maize Inflorescence Culture.- 130. Shoot Meristem Culture.- 131. Establishment and Culture of Maize Endosperm.- 132. In vitro Pollen Germination.- 133. Axillary Bud in vitro Culture: Asexual Propagation of Maize.